ISSN:
1573-5028
Keywords:
Indica rice
;
IRRI breeding line
;
PEG-mediated protoplast transformation
;
hygromycin phosphotransferase
;
phosphinothricin acetyltransferase
;
herbicide resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The commercially important Indica rice cultivar Oryza sativa cv. IR72 has been transformed using direct gene transfer to protoplasts. PEG-mediated transformation was done with two plasmid constructs containing either a CaMV 35S promoter/HPH chimaeric gene conferring resistance to hygromycin (Hg) or a CaMV 35S promoter/BAR chimaeric gene conferring resistance to a commercial herbicide (Basta) containing phosphinothricin (PPT). We have obtained so far 92 Hgr and 170 PPTr IR72 plants from protoplasts through selection. 31 Hgr and 70 PPTr plants are being grown in the greenhouse to maturity. Data from Southern analysis and enzyme assays proved that the transgene was stably integrated into the host genome and expressed. Transgenic plants showed complete resistance to high doses of the commercial formulations of PPT.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00046447
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