ISSN:
1423-0127
Keywords:
Patch clamp
;
Single-channel recording
;
Whole cell recording
;
Cytosolic calcium
;
Microspectrofluorimetry
;
Indo 1
;
Prostate
;
5α-Reductase
;
Protein kinase C
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The lipidosterolic extract from the saw palmettoSerenoa repens (LSESr) is commonly used for medical treatment of benign prostatic hypertrophia due to its ability to inhibit 5α-reductase which permits the conversion of testosterone to dihydrotestosterone, the active androgen on prostate cell proliferation. However, the complete action mechanism of LSESr is still unknown. Several lines of evidence suggest that, in addition to inhibition of 5α-reductase, it may interfere with the action of prolactin (PRL). We therefore investigated a possible interference of this plant extract with another hormone that controls prostate gland growth, PRL. As the action mechanism of PRL is now fully documented in Chinese hamster ovary cells expressing the PRL receptor, we have conducted our experiments on these cells. In this study, using electrophysiological (whole-cell recording and single-channel recording), microspectrofluorimetric and biochemical techniques, we show that LSESr (1–30 µg/ml) reduced the basal activity of a K+ channel and of protein kinase C (PKC) in CHO cells. In addition, pretreatment of the cells with 1–10 µg/ml LSESr for 6–36 h abolished the effects of PRL on [Ca2+]i, K+ conductance and PKC. LSESr may block PRL-induced prostate growth by inhibiting several steps of PRL receptor signal transduction. LSESr may also be useful for diseases implicating PRL.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02255223
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