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  • 1
    Electronic Resource
    Electronic Resource
    Organization, promoter analysis and transcriptional regulation of the Staphylococcus xylosus xylose utilization operon (1991)
    Springer
    Molecular genetics and genomics 227 (1991), S. 377-384 
    Details
    ISSN: 1617-4623
    Keywords: Xylose utilization ; Xylose isomerase ; Promoter structure ; Staphylococci ; xyl regulon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Staphylococcus xylosus xyl genes were cloned in Staphylococcus carnosus by complementation to xylose utilization. Xylose isomerase assays under inducing (xylose present) and non-inducing (xylose absent) conditions indicated the presence of a regulated xylA gene on the recombinant plasmid. The nucleotide sequence (4520 bases) revealed three open reading frames with the same polarity. They were identified by sequence homologies as xylR, encoding the Xyl repressor, xylA, encoding xylose isomerase and xylB, encoding xylulokinase. Primer extension analyses indicated constitutive transcription of xylR and xylose-inducible transcription of xylA. Promoter consensus sequences were found upstream of both transcriptional start sites. A transcriptional terminator between xylR and xylA separates the different transcriptional units. Potential regulatory elements were identified by sequence analysis and suggest a repressor-operator mechanism for the regulation of xylAB expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273926
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Regulation of the Bacillus subtilis W23 xylose utilization operon : interaction of the Xyl repressor with the xyl operator and the inducer xylose (1992)
    Springer
    Molecular genetics and genomics 232 (1992), S. 415-422 
    Details
    ISSN: 1617-4623
    Keywords: xyl regulation ; Crude protein extract ; Gel mobility shift assay ; In situ footprinting ; Induction studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A crude protein extract of Bacillus subtilis W23 contains a sequence-specific DNA binding activity for the xyl operator as detected by the gel mobility shift assay. A xylR determinant encoded on a multicopy plasmid leads to increased expression of this binding activity. In situ footprinting analysis of the protein-DNA complex in a polyacrylamide gel shows that the xyl operator is sequence-specifically bound and protected from cleavage by copper-phenanthroline at 26 phosphodiester bonds on each strand. Quantitative competition assays for repressor binding reveal that a 25 by synthetic xyl operator cloned into a polylinker is bound with the same affinity as the operator in the wild-type xyl regulatory region. This confirms that no additional sites in the wild-type sequence contribute to repressor binding. The xyl operator consists of ten palindromic base pairs flanking five central non-palindromic base pairs. A mutational analysis shows that the sequence of the central base pairs contributes to recognition by the repressor protein and that the spacing of the palindromic elements is crucial for repressor binding. An operator half site is not bound by the repressor. In vivo and in vitro induction studies suggest that, of several structurally similar sugars, xylose is the only molecular inducer of the Xyl repressor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266245
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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