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Allergens in raw and roasted hazelnuts (Corylus avellana) and their cross-reactivity to pollen (2000)

Müller, U. ; Lüttkopf, D. ; Hoffmann, A. ; [et al.]

Springer

European food research and technology 212 (2000), S. 2-12

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ISSN: 1438-2385

Keywords: Key words Hazelnut allergens ; Cross-reactivity ; Cross-reactive carbohydrate determinants ; Heat-stable allergens ; Rat basophil leukaemia cell assay

Source: Springer Online Journal Archives 1860-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract Hazelnuts (HNs), widely distributed in sweets, provoke one of the most frequent pollen-associated food allergies. In this study HN allergens were investigated and immunologically characterized, focussing on their heat stability and cross-reactivity with known allergenic structures. Therefore, 27 sera from HN-allergic patients and 28 sera from children with positive CAP classes for HN and birch pollen were submitted to immunoblot and immunoblot inhibition. The major HN allergen was found to present a molecular mass of about 17–18 kDa, and to share IgE epitopes with Bet v 1, the major birch pollen allergen. This allergen was recognized by IgE of 93% of our HN-allergic patients and by 79% of sensitized children. A 48 kDa glycoprotein was identified as a minor HN allergen with cross-reactive carbohydrate determinants. The major N-glycan species was determined by matrix-assisted laser desorption mass spectrometry to be Man3XylGlcNAc2. IgE binding to this protein was detected in sera of 41% of the allergic patients and 61% of the children. Partial N-terminal sequencing demonstrated similarity to legume storage proteins. The IgE reactivity of this structure was partially resistant to heating. The rat basophil leukaemia cell mediator release assay was used for estimation of cross-sensitization between HN and birch pollen, confirming the finding that HNs contain a heat-resistant allergenicity without cross-reactivity to birch pollen allergens.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002170000245

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Ein Beitrag über Oxide vom Typ AMO4 (A = Ti3+, Cr3+; M = Nb5+, Ta5+) (1992)

Petersen, A. ; Müller-Buschbaum, Hk.

Weinheim : Wiley-Blackwell

Zeitschrift für anorganische Chemie 609 (1992), S. 51-54

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ISSN: 0044-2313

Keywords: Titanium ; chromium ; niobium ; tantalum ; oxide ; structure ; Chemistry ; Inorganic Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: A Contribution on Ternary Oxides of the AMO4-Type (A = Ti3+, Cr3+; M = Nb5+, Ta5+)CrNbO4, CrTaO4, TiNbO4, and TiTaO4 were prepared by CO2-laser technique. X-ray single crystal investigations show a random distribution of the metal ions in Rutil type structure, space group D4h14-P42/mnm. Calculations of the free energy of reaction between Ti2O3 and Nb2O5 show higher stability of TiO2 beside NbO2. In TiNbO4 both metals exhibit the oxidation state +4.

Notes: CrNbO4, CrTaO4 TiNbO4 und TiTaO4 wurden einkristallin mit CO2-Lasertechnik dargestellt und röntgenographisch untersucht. Sie kristallisieren mit statistischer Verteilung der Metallionen im Rutiltyp, Raumgruppe D4h14-P42/mnm. Berechnungen der freien Reaktionsenthalpie zeigen, daß Ti2O3 nicht neben Nb2O5vorliegt. In TiNbO4 haben beide Metallionen die Oxidationsstufe +4.

Additional Material: 3 Tab.