ISSN:
1434-0879
Keywords:
Instillation therapy
;
Superficial bladder cancer
;
Antitumor activity
;
Cytotoxic effect
;
Urinary pH
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The effects of pH on the antitumour activity of six cytostatics—cisplatin, doxorubicin, ethoglucid, mitomycin C, thiotepa and VM-26 — used in intravesical instillations were tested in vitro. The activity of cytostatics was determined on the Walker 256 carcinosarcoma cell line by using ATP-bioluminescence method. Cytostatics were incubated at different pH's (5.0–7.4) for 2 and 4h. The activity of most of the cytostatics was not affected by pH, but mitomycin C had somewhat lower activity at pH 5.0 at 4 h and cisplatin at pH 6–7 at 2 and 4h. Because the pH of normal urine is within the range used in this test, the use of a buffer solution during the intravesical instillation therapy is not necessary. However, according to our clinical experience, buffering is recommended to avoid chemical cystitis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00571751
Permalink
