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  • Insulin  (1)
  • stimulation of insulin release by cyclic-AMP and tolbutamide  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Diabetologia 9 (1973), S. 251-254 
    ISSN: 1432-0428
    Keywords: Human insulinoma ; culture of dissociated tumorβ-cells ; insulin release in culture ; stimulation of insulin release by cyclic-AMP and tolbutamide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Tumor cells from two human insulinomas were maintained in culture for more than 6 months. The cultured cells synthesized and secreted proinsulin and insulin; and responded to cyclic AMP (1 mM) and tolbutamide (1 mM) stimulation with increased insulin release. In culture, these cells did not respond to stimulation by high concentrations of glucose (300 mg%) or by glucagon (10 μg/ml).
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Diabetologia 4 (1968), S. 68-70 
    ISSN: 1432-0428
    Keywords: Insulin ; proinsulin ; biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Après incubation de tranches de pancréas d'embryon de veau, la leucine-H3 est incorporée dans une fraction protéique qui semble avoir les propriétés d'une “proinsuline”. Cette fraction protéique est de taille supérieure à l'insuline, possède l'immunoréactivité propre à l'insuline, et après traitement limité par la trypsine elle est transformée en un peptide semblable à l'insuline.
    Abstract: Zusammenfassung Die Inkubierung von Dünnschnitten des fötalen Rinder-Pankreas in Gegenwart vom H3- Leucin ergab einen Einbau dieser Amminosäure in eine Eiweißfraktion, die die Eigenschaften eines, Pro-Insulins' aufwies. Das Molekulargewicht dieser Eiweißfraktion war größer als dasjenige des Insulins; sie besaß die Immunreaktivität des Insulins und konnte durch teilweisen Abbau mit Trypsin in ein insulinähnliches Peptid umgewandelt werden.
    Notes: Summary Incubation of fœtal bovine pancreas slices resulted in the incorporation of3H-leucine into a protein fraction which appeared to have the properties of a ‘proinsulin’. This protein fraction was larger in size than insulin, possessed the immunoreactivity of insulin and was converted by limited trypsin treatment to a peptide similar to insulin.
    Type of Medium: Electronic Resource
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