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  • 1
    Electronic Resource
    Electronic Resource
    DNA vaccination using coexpression of cytokine genes with a bacterial gene encoding a 60-kDa heat shock protein (2000)
    Springer
    Medical microbiology and immunology 189 (2000), S. 97-104 
    Details
    ISSN: 1432-1831
    Keywords: Key words DNA vaccination ; Bacterial heat shock protein ; Interleukin-2 ; Interleukin-4 ; Interferon-γ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Coexpression of cytokine genes together with antigen-encoding genes in DNA vaccination vectors can increase humoral and cellular immune responses and may steer them in a Th1 or Th2 direction. In this study, the modulatory effect of interleukin (IL)-2, IL-4, and interferon (IFN)-γ coexpressed with the 60-kDa heat shock protein (Hsp60) of Yersinia enterocolitica O:8 (Y-Hsp60) was studied. DNA vaccination with y-hsp60 evoked specific humoral and cellular immune responses as well as reduction of the splenic bacterial load upon challenge with Y. enterocolitica in a mouse infection model. Coexpression of IL-2 or IFN-γ enhanced Y. enterocolitica-specific total IgG (P 〈 0.05) and IgG2a antibody responses. Coexpression of IFN-γ also improved the proliferative T cell responses upon stimulation with Y-Hsp60. A reduction of the splenic bacterial load as compared with the plasmid encoding Y-Hsp60 only was found for the IFN-γ coexpressing vector. Thus, coexpression of cytokine genes such as IFN-γ in DNA vaccination vectors might improve immunity and help to overcome the side effects of standard adjuvants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004300000047
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  • 2
    Electronic Resource
    Electronic Resource
    Wortmannin blocks Yersinia invasin-triggered internalization, but not interleukin-8 production by epithelial cells (1998)
    Springer
    Medical microbiology and immunology 187 (1998), S. 53-60 
    Details
    ISSN: 1432-1831
    Keywords: Key wordsYersinia enterocolitica ; Wortmannin ; PI3-kinase ; Interleukin-8 ; invasin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In response to bacterial infection epithelial cells up-regulate expression and secretion of proinflammatory cytokines. Previous work from our laboratory showed that basolateral infection of polarized T84 cells with Yersinia enterocolitica induces interleukin-8 (IL-8) secretion in the absence of significant invasion. Here we studied Y. enterocolitica-induced IL-8 secretion by epithelial HeLa cells as a function of Yersinia invasion or adhesion. For this purpose we tried to separated induction of IL-8 secretion from invasion by treating HeLa cells with signal transduction inhibitors prior to infection. While staurosporin and genistein inhibited both Yersinia invasion and Yersinia-triggered IL-8 secretion, wortmannin, an inhibitor of the phosphatidylinositol-3-phosphate kinase (PI3-K), blocked invasion of Y. enterocolitica into HeLa cells but did not show any effect on IL-8 secretion. These results suggest that Yersinia adhesion might be sufficient to induce IL-8 secretion by epithelial cells. Further analysis demonstrated the requirement of the Yersinia invasion locus inv for adhesion-mediated induction of IL-8 secretion. Thus, HeLa cells infected with an E. coli strain expressing the Y. enterocolitica inv locus induced IL-8 secretion in the presence and absence of wortmannin. Reverse transcription-polymerase chain reaction analysis revealed that adhesion of inv-expressing Y. enterocolitica or E. coli results in the transcriptional activation of the IL-8 gene. These results suggest that Y. enterocolitica adhesion to host cells via Inv activates de novo synthesis and secretion of IL-8.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004300050074
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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