ISSN:
1573-5060
Keywords:
Pisum sativum
;
field pea
;
exogenous DNA
;
legume nodulation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary The nodulation response of a non-nodulating field pea line (Pisum sativum L., PRL-H722) following treatment with DNA from an effectively nodulating cultivar (Trapper) has been investigated. DNA was introduced to the non-nodulating cultivar via imbibition and germination of seeds in DNA solutions under aseptic conditions. The DNA preparations used were 〉5×106 Daltons, had a smooth melting profile showing ≥40% hyperchromicity with a Tm=70–72°C in 0.1×SSC0 buffer, and 260/280 nm ratio of 〉1.8. A significant correction (ϰ2(I d.f.) = 2032**) of the mutant phenotype was observed in seeds treated with wild-type DNA. The nodulation response in treated plants was not observed in subsequent generations suggesting that the change observed did not involve integration of genetic information into the genome of germ line cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00036877
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