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  • Inverse PCR  (1)
  • Key words: Laparoscopic — Nissen Fundoplication — Diffusing capacity — Lung function — Cough — Gastroesophageal reflux  (1)
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Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 10 (1996), S. 1171-1175 
    ISSN: 1432-2218
    Keywords: Key words: Laparoscopic — Nissen Fundoplication — Diffusing capacity — Lung function — Cough — Gastroesophageal reflux
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: An effort was made to assess the respiratory outcomes of laparoscopic Nissen fundoplication (LNF). Methods: Prospective follow-up of 69 patients undergoing LNF for gastroesophageal reflux disease. Outcomes included pulmonary function testing, 24-h pH recording, esophageal manometry, and symptom assessment. Results: There was an improvement (p 〈 0.0001) in heartburn and cough scores. There was a significant fall in spirometry (p 〈 0001), diffusing capacity (p 〈 0.0001), and respiratory muscle strength (p 〈 0.0001) 36 h after surgery, which had returned to baseline by 1 month. At 6 months, the patients (n= 16) with impaired preoperative diffusing capacity showed improvement (17.8 ± 3.7 to 19.8 ± 4.6 ml/min/mmHg, p= 0.0245). Conclusion: Patients undergoing LNF have impaired gas exchange before surgery which tends to improve 6 months after surgery. There is an early reversible impairment in respiratory function due to diaphragm dysfunction. Patients with a preoperative 1-s forced expired volume 〉 1.5, or 50% predicted, are unlikely to develop signficant early respiratory complication.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Restriction enzyme ; Methylase selection ; Endo-blue method ; N4 methylase ; Inverse PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract AvaI andBsoBI restriction endonucleases are isoschizomers which recognize the symmetric sequence 5′CYCGRG3′ and cleave between the first C and second Y to generate a four-base 5′ extension. TheAvaI restriction endonuclease gene (avaIR) and methylase gene (avaIM) were cloned intoEscherichia coli by the methylase selection method. TheBsoBI restriction endonuclease gene (bsoBIR) and part of theBsoBI methylase gene (bsoBIM) were cloned by the “endo-blue” method (SOS induction assay), and the remainder ofbsoBIM was cloned by inverse PCR. The nucleotide sequences of the two restriction-modification (RM) systems were determined. Comparisons of the predicted amino acid sequences indicated thatAvaI andBsoBI endonucleases share 55% identity, whereas the two methylases share 41% identity. Although the two systems show similarity in protein sequence, their gene organization differs. TheavaIM gene precedesavaIR in theAvaI RM system, while thebsoBIR gene is located upstream ofbsoBIM in theBsoBI RM system. BothAvaI andBsoBI methylases contain motifs conserved among the N4 cytosine methylases.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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