ISSN:
1435-1536
Keywords:
Inverse microemulsion polymerization
;
microemulsion
;
protein immobilization
;
latex
;
surface modification
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract This paper deals with the immobilization of alkaline phosphatase by physical entrapment within colloidal particles produced by inverse microemulsion polymerization. Functionality has been imparted to the nanoparticle surface by copolymerization of acrylamide (the main monomer),N,N′-methylene-bis-acrylamide (the cross-linking agent) with eitherN-acryloyl-1,6-diaminohexane (an amine promoter) or acrylic acid (a carboxylic acid promoter). The effect of the functional comonomers on the size and zeta potential of the reactive latexes has been studied. Integrity of the immobilized enzyme has been ascertained from its catalytic activity towards hydrolysis ofp-nitrophenylphosphate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00652472
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