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  • 1
    ISSN: 1573-1561
    Keywords: Sweet potato weevil ; Cylas formicarius elegantulus ; Coleoptera ; Curculionidae ; Ipomoea batatas ; sweet potato volatiles ; sesquiterpenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A dual-choice olfactometer was developed to study the responses of sweet potato weevils,Cylas formicarius elegantulus (Summers), to volatiles from the sweet potato,Ipomoea batatas (L.) Lam. Both males and females were attracted by volatiles from sweet potato leaves and a methylene chloride leaf extract. Females, but not males, responded to volatiles from storage roots and a methylene chloride root extract. Leaves and storage roots from four sweet potato cultivars (Centennial, Jewel, Resisto, and Regal) were attractive to female weevils; however, the attractant response varied with cultivar. GC profiles from leaf and root extracts, and GC-MS analysis of leaf extract, for Jewel cultivar enabled the volatile peaks to be identified as sesquiterpenes.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Catnip ; Nepeta cataria ; caffeoyltartronic acid ; lubber grasshopper ; Romalea guttata ; catechol secretions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Adults of the lubber grasshopper (Romalea guttata) secrete increased amounts of catechol from their defensive glands when fed diets containing only catnip leaves (Nepeta cataria). Model compound bioassays showed that these insects were able to sequester and biomagnify simple phenols, such as catechol and hydroquinone, in their defense gland secretions. Excessive catechol secretions from caffeic acid-fortified diets indicated metabolic pathways exist to perform efficiently more complex biochemical conversions. Reverse-phase HPLC of methanol extracts of catnip revealed only one major caffeoyl-polyphenol as a possible precursor for the observed elevated catechol secretions, when this plant is fed to lubbers. The compound was shown to be caffeoyltartronic acid (CTA). During analysis of CTA by probe-MS or gas chromatography (of its silylated derivative), CTA decomposed by loss of carbon dioxide to form caffeoylglycolic acid (CGA), making identification by these methods ambiguous. Only fast atom bombardment mass spectrometry (FAB-MS, negative mode) gave a true molecular weight. Groundivy (Glecoma hederacea), a relative of catnip, was also shown to contain CTA. The mung bean (Phaseolus radiatus=Vigna radiata), a species totally unrelated to catnip, is the only other reported plant source of CTA. Catnip leaves were found to contain about twice as much CTA as mung bean leaves.
    Type of Medium: Electronic Resource
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