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  • 1
    Electronic Resource
    Electronic Resource
    Anthocyanin-flavone copigmentation in bluish purple flowers of Japanese garden iris (Iris ensata Thunb.) (1997)
    Springer
    Euphytica 98 (1997), S. 163-167 
    Details
    ISSN: 1573-5060
    Keywords: Iris ensata ; copigmentation ; bluing effect ; flower color breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The in vitro identification of copigmentation was carried out using anthocyanins, such as malvidin 3RGac5G, petunidin 3RGac5G and delphinidin 3RGac5G and the flavone isovitexin. These are major pigments of Iris ensata. These anthocyanins brought about copigmentation, i.e., the bathochromic shift (bluing effect) of visible λ max due to increased concentrations of isovitexin, and 32 to 35 nm were estimated as the magnitude (Δλ max) of each shift. In addition, the absorption spectrum of 0.1 mM malvidin 3RGac5G, 0.07 mM petunidin 3RGac5G and 0.7 mM isovitexin mixture closely matched those of the fresh outer perianths of the bluish purple cultivars, ‘Suiten-isshoku’, ‘Hekikai’ and ‘Yakonotama’ which belong to the malvidin 3RGac5G - petunidin 3RGac5G type of I. ensata. Therefore, these results indicated that the bluing effect on the flower color of the bluish purple cultivars of this species was caused at least in part by the copigmentation between these anthocyanins and the flavone isovitexin. For the copigment effects of isovitexin among malvidin 3RGac5G, petunidin 3RGac5G and delphinidin 3RGac5G, λ max and Δλ max of delphinidin 3RGac5G was slightly higher than those of malvidin 3RGac5G or petunidin 3RGac5G, and the copigmentation of delphinidin 3RGac5G was characterized by a higher concentration of isovitexin. Finally, the breeding for blue flowers due to copigmentation of delphinidin 3RGac5G with isovitexin in I. ensata was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003152813333
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Tissue edema and loss of tracer microspheres in infarcted myocardium (1983)
    Springer
    Basic research in cardiology 78 (1983), S. 124-130 
    Details
    ISSN: 1435-1803
    Keywords: myocardial infarction ; tissue edema ; tracer microspheres ; microsphere loss ; regional myocardial blood flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Microsphere loss from the ischemic myocardium was studied in a canine model after 2 and 7 days coronary occlusion. 10 million tracer microspheres (TM) of 7–10 μm diameter were injected into the left atrium before and 15 minutes after left anterior descending coronary artery (LAD) occlusion. To determine the tissue water content, dried tissue weight was measured after desiccation. TM content in the nonoccluded left circumflex coronary artery (LCX) area was unchanged before and after coronary occlusion. In the infarcted area, microsphere loss was maximum at the low flow endocardial region averaging 27% and 42% in 2 and 7 days occlusion, respectively. An inverse linear correlation between TM loss and regional blood flow in 2 (r=−0.82, p〈0.05) and 7 (r=−0.96, p〈0.01) days coronary occlusion was noted. Water content was increased in the ischemic endocardium by 4.1 and 5.7% in 2 and 7 days occlusion, thus approximately 17% of TM loss was attributed to tissue edema. These results suggest that there is an ischemia-dependent reduction of TM in the ischemic myocardium and that microsphere migration participates in the major part of TM loss. Thus flow measurements by TM may be invalid when this compound is injected during the active phase of microsphere migration or during water content alterations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01906666
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  • 3
    Electronic Resource
    Electronic Resource
    Linear relationship between perfusion area and infarct size (1981)
    Springer
    Basic research in cardiology 76 (1981), S. 438-442 
    Details
    ISSN: 1435-1803
    Keywords: perfusion area ; infarct size ; tracer microspheres ; autoradiography ; tetrazolium staining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The purpose of this study was to develop a technique measuring the perfusion area of the coronary artery preocclusively and to study the relationship between the perfusion are aand infarct size.125I tracer microspheres were selectively injected into the left circumflex coronary artery (LCX) preocclusively, and then the LCX was ligated 48 hours later the heart was removed, rapidly frozen, and 50-μ transverse sections were obtained from base, middle and apex of the canine left ventricle, and used for autoradiography to measure perfusion area and for tetrazolium staining to measure infarct size. Dogs were divided into 2 groups: group 1 in which the main trunk of the LCX was occluded to produce large infarct (n=10) and group 2 in which the distal branch of the LCX was occluded to produce small infarct (n=10). There was a linear correlation between the perfused and infarcted area regardless of a size or location of the perfusion area involved. These results indicate that the extent of infarction is directly proportional to the perfusion area and is not altered by the location in the ventricle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01908338
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    P-coumaroyl glycosides of cyanidin and peonidin in the flowers of Japanese garden iris, Iris ensata Thunb. (1993)
    Springer
    Euphytica 74 (1993), S. 47-50 
    Details
    ISSN: 1573-5060
    Keywords: Iris ensata ; Japanese garden iris ; anthocyanins ; cyanidin 3RGac5G ; peonidin 3RGac5G ; flower breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The anthocyanins in outer perianths of seven varieties and one telotrisomic line of Japanese garden iris, Iris ensata Thunb., were analysed by high-performance liquid chromatography procedures. Two new anthocyanins, peonidin 3RGac5G and cyanidin 3RGac5G were putatively detected in this species, and peonidin 3RGac5G was the major anthocyanin for all the varieties except ‘Telotri-1’ and cyanidin 3RGac5G the major anthocyanin for ‘Telotri-1’. Moreover, cyanidin 3RGac5G and peonidin 3RGac5G are useful anthocyanins for the breeding of red and magenta flowers. Finally, the breeding strategy for new flower varieties using cyanidin 3RGac5G and peonidin 3RGac5G was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033766
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    Paper (German National Licenses)
    Fulltext
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