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  • Isoenzyme  (1)
  • Key words Hordeum vulgare  (1)
  • Key words Molecular marker  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Analysis of the transgenic tobacco plants expressing Panicum miliaceum aspartate aminotransferase genes (2000)
    Springer
    Plant cell reports 19 (2000), S. 598-603 
    Details
    ISSN: 1432-203X
    Keywords: Key words Aspartate aminotransferase ; Phosphoenolpyruvate carboxylase ; Isoenzyme ; Transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Expression of Panicum miliaceum L. (proso millet) mitochondrial and cytosolic aspartate aminotransferase (mAspAT and cAspAT, respectively) genes in transgenic tobacco plants (Nicotiana tabacum) and their influences on protein synthesis were examined. The mAspAT- or cAspAT-transformed plants had about threefold or 3.5-fold higher AspAT activity in the leaf than non-transformed plants, respectively. Interestingly, the leaves of both transformed plants had increased levels of phosphoenolpyruvate carboxylase (PEPC) and transformed plants with cAspAT also had increased levels of mAspAT in the leaf. These results suggest that the increased expression of Panicum cAspAT in transgenic tobacco enhances the expression of its endogenous mAspAT and PEPC, and the increased expression of Panicum mAspAT enhances the expression of its endogenous PEPC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050779
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Map construction of sequence-tagged sites (STSs) in barley (Hordeum vulgare L.) (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 937-946 
    Details
    ISSN: 1432-2242
    Keywords: Key words Hordeum vulgare ; Linkage map ; Recombinant inbred ; Sequence-tagged site (STS)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In order to identify sequence-tagged sites (STSs) appropriate for recombinant inbred lines (RILs) of barley cultivars ‘Azumamugi’ × ‘Kanto Nakate Gold’, a total of 43 STS primer pairs were generated on the basis of the terminal sequences of barley restriction fragment length polymorphism (RFLP) clones. Forty one of the 43 primer pairs amplified PCR products in Azumamugi, Kanto Nakate Gold, or both. Of these, two showed a length polymorphism and two showed the presence or absence of polymorphism between the parents. PCR products of the remaining 37 primers were digested with 46 restriction endonucleases, and polymorphisms were detected for 15 primers. A 383.6-cM linkage map of RILs of Azumamugi×Kanto Nakate Gold was constructed from the 19 polymorphic STS primer pairs (20 loci) developed in this study, 45 previously developed STS primer pairs (47 loci), and two morphological loci. Linkage analysis and analysis of wheat-barley chromosome addition lines showed that with three exceptions, the chromosome locations of the STS markers were identical with those of the RFLP markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051153
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of random amplified polymorphic DNA (RAPD) markers linked to the v locus in barley, Hordeum vulgare L. (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 637-642 
    Details
    ISSN: 1432-2242
    Keywords: Key words Molecular marker ; v locus (kernel row type) ; Hordeum vulgare L. ; RAPD ; Recombinant backcross line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Recombinant backcross lines of barley were produced from a cross between Kanto Nakate Gold (KNG; two-rowed) and Azumamugi (AZ; six-rowed) after backcrosses of F1 plants with AZ as the recurrent parent. Each of these lines had an introgressed segment from chromosome 2 of KNG. Two recombinant backcross lines, L1 and M3-13, were used for an initial screening of polymorphism. After screening a total of 888 oligonucleotides as arbitrary primers, we identified eight random amplified polymorphic DNAs (RAPDs) between backcross lines and AZ. Among the RAPD fragments, CMNA-38700 was linked to the v locus with a recombination frequency of zero, while OPJ-09850 and OPP-02700 were linked to the v locus at a map distance of 1.4 cM. Thus, the three RAPD markers were clustered around the v locus since the lengths of introgressed chromosomal segments in the L1 and M3-13 lines were no less than 38 cM. The other five RAPD fragments that we identified were not linked to the v locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050606
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    Paper (German National Licenses)
    Fulltext
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