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Prognostic significance of dysplastic features of hematopoiesis in patients with de novo acute myelogenous leukemia (1997)

Kahl, C. ; Florschütz, A. ; Müller, G. ; [et al.]

Springer

Annals of hematology 75 (1997), S. 91-94

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ISSN: 1432-0584

Keywords: Key words AML ; Trilineage dysplasia ; Dysgranulopoiesis ; Dyserythropoiesis ; Dysmegakaryopoiesis

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract The detection of dysplastic features of hematopoiesis in de novo acute myeloid leukemia (AML) by light microscopy is defined as AML with trilineage myelodysplasia (AML/TLMD). The prognostic relevance of these dysplastic features for patients with de novo AML remains unclear. In order to evaluate the role of dysplasia in de novo AML, bone marrow aspirates from 69 patients were analyzed prospectively and investigated separately for erythropoiesis, granulopoiesis and megakaryopoiesis by three independent investigators. The overall complete remission (CR) rate was 48.8% and partial remission (PR) or nonresponders consituted 52.2% of the patients investigated. The median overall survival time was 5 months with a disease-free interval of 3.5 months for all patients. Dysgranulopoiesis (DysG) was observed in 30.4%, dysmegakaryopoiesis (DysM) in 50.7%, and dyserythropoiesis (DysE) in 43.5%. Of all patients, 26.0% showed trilineage dysplastic features and were thus classified as AML/TLMD. A significantly worse prognosis (Kaplan-Meyer plot, Student's t–test) was calculated for those patients with detection of only DysG (p=0.002), DysM (p=0.02), DysE (p=0.04) as compared with patients without any dysplastic signs. An unfavorable karyotype was correlated with patients showing DysG (P=0.02) and DysM (P=0.04). For these patients with an unfavorable karyotype, the occurrence of any dysplastic features had no additional prognostic impact. Dysplastic features (DysG, DysM, DysE) seem to be an important prognostic factor in de novo AML correlating with short overall survival. DysG and DysM correlated well with the appearance of unfavorable chromosomal abnormalities. It may be reasonable to assume that patients with dysplastic features should be considered for more aggressive treatment schedules at the time of diagnosis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002770050320

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Biological activity of prostate-specific antigen isolated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution (1995)

Tessmer, Uwe ; Quack, Thomas ; Donn, Folkert ; [et al.]

Weinheim : Wiley-Blackwell

Electrophoresis 16 (1995), S. 793-799

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ISSN: 0173-0835

Keywords: Prostate-specific antigen ; Semenogelin ; Isolation ; Sodium dodecyl sulfate-polyacrylamide gel electrophoresis ; Complexes ; Enzyme activity ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Human prostate-specific antigen (PSA), a 33 kDa kallikrein-like serine protease, occurring in the prostate, in seminal plasma and in blood, was prepared under nonreducing conditions in an enzymatically active form from seminal plasma by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by fast copper staining, electroelution from gel slices and dialysis against isotonic phosphate-buffered saline (PBS). Enzymatic activity was demonstrated for the first time directly by cleavage of semenogelin, one of the biological substrates of PSA, isolated by the same procedure, i.e. SDS-PAGE and electroelution, but from seminal vesicle fluid. The purified PSA formed SDS-stable complexes with the two major extracellular protease inhibitors in blood, α1-antichymotrypsin (α1-ACH) and α2-macroglobulin (α2-M). PSA isolated under reducing conditions was enzymatically inactive and could not bind to the protease inhibitors α1-ACH and α2-M.

Additional Material: 6 Ill.