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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of Penicillium chrysogenum with a dominant selectable marker (1988)
    Springer
    Current genetics 13 (1988), S. 377-382 
    Details
    ISSN: 1432-0983
    Keywords: Penicillium chrysogenum ; Oligomycinresistance ; Transformation ; Isopenicillin N synthetase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have cloned a mutant oligomycin resistance allele of the mitochondrial ATP synthase subunit 9 gene from the filamentous fungus Penicillium chrysogenum. The gene was isolated using the equivalent gene from Aspergillus nidulans as a hybridisation probe. Using the cloned gene it is possible to select for oligomycin resistance in P. chrysogenum transformation experiments. This transformation system was used to introduce further copies of the P. chrysogenum isopenicillin N synthetase gene, which were stably maintained without selection. An assessment of the frequency with which homologous integration occurs was also made. With this system, it should prove possible to transform any strain of P. chrysogenum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365658
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Glucose-induced inactivation of isocitrate lyase in Aspergillus nidulans (1994)
    Springer
    Archives of microbiology 162 (1994), S. 409-413 
    Details
    ISSN: 1432-072X
    Keywords: Aspergillus nidulans ; Catabolite inactivation ; Isocitrate lyase ; creA Gene ; Gene regulation ; Fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The existence of a second mechanism of catabolite control of isocitrate lyase of Aspergillus nidulans, in addition to the carbon catabolite repression phenomenon recently reported was analysed. Isocitrate lyase was rapidly and specifically inactivated by glucose. The inactivation was irreversible at all stages in the presence of cycloheximide, showing that reactivation depends on de novo protein synthesis. In addition, analysis of glucose-induced inactivation of isocitrate lyase in a creA d-30 strain showed that the creA gene is not involved in this process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282105
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Glucose-induced inactivation of isocitrate lyase in Aspergillus nidulans (1994)
    Springer
    Archives of microbiology 162 (1994), S. 409-413 
    Details
    ISSN: 1432-072X
    Keywords: Key words     Aspergillus nidulans ; Catabolite ; inactivation ; Isocitrate lyase ; creA Gene ; Gene ; regulation ; Fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      The existence of a second mechanism of catabolite control of isocitrate lyase of Aspergillus nidulans, in addition to the carbon catabolite repression phenomenon recently reported was analysed. Isocitrate lyase was rapidly and specifically inactivated by glucose. The inactivation was irreversible at all stages in the presence of cycloheximide, showing that reactivation depends on de novo protein synthesis. In addition, analysis of glucose-induced inactivation of isocitrate lyase in a creA d -30 strain showed that the creA gene is not involved in this process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050158
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Amplification of the isopenicillin N synthetase gene in a strain of Penicillium chrysogenum producing high levels of penicillin (1989)
    Springer
    Molecular genetics and genomics 216 (1989), S. 492-497 
    Details
    ISSN: 1617-4623
    Keywords: Gene amplification ; Isopenicillin N synthetase ; β-Lactams ; Penicillium chrysogenum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The isopenicillin N synthetase (IPNS) gene has been isolated from wild-type Penicillium chrysogenum and used as a probe to detect the equivalent gene on Southern blots of genomic DNA from a mutant producing high levels of penicillin. The IPNS gene in this strain is contained within a region of DNA of wild-type restriction pattern that extends for at least 39 kb and is present at between 8 and 16 copies. The steady state level of IPNS mRNA in the mutant producing high levels of penicillin is between 32-and 64-fold of that of the wild type, suggesting that the rate of transcription of some or all of the copies has been increased. In addition we have also shown that both the IPNS mRNA and enzyme is present throughout the growth phase in both strains under the culture conditions used. IPNS enzyme activity is greatly increased in the strain with the high penicillin titre.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334395
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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