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  • 1
    ISSN: 1432-1432
    Keywords: Microsporidians ; Glugea plecoglossi ; Eukaryotes ; Mitochondrion-lacking protozoa ; Elongation factor 1α ; Protein phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Partial regions of the mRNA encoding a major part of translation elongation factor 1α (EF-1α) from a mitochondrion-lacking protozoan,Glugea plecoglossi, that belongs to microsporidians, were amplified by polymerase chain reaction (PCR) and their primary structures were analyzed. The deduced amino acid sequence was highly divergent from typical EF-1α's of eukaryotes, although it clearly showed a eukaryotic feature when aligned with homologs of the three primary kingdoms. Maximum likelihood (ML) analyses on the basis of six different stochastic models of amino acid substitutions and a maximum parsimony (MP) analysis consistently suggest that among eukaryotic species being analyzed,G. plecoglossi is likely to represent the earliest offshoot of eukaryotes. Microsporidians might be the extremely ancient eukaryotes which have diverged before an occurrence of mitochondrial symbiosis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 11 (1997), S. 451-455 
    ISSN: 0268-2605
    Keywords: imposex ; Japanese sea snails ; organotin pollution ; tributyltin (TBT) ; triphenyltin (TPT) ; Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A country-wide survey and laboratory experiments on imposex (a superimposition of male sex organs on female sea snails) in Japanese sea snails were carried out: 38 species were observed to be affected by imposex. For the rock shell, Thais clavigera, the percentage occurrence of imposex was 100% at almost all sites surveyed. The degree of imposex seemed to be positively correlated with the concentrations of tributyltin (TBT) and triphenyltin (TPT) in the species. The results of laboratory experiments showed that imposex in T. clavigera was initiated and promoted by both TBT and TPT. Many oviduct-blocked individuals, which were thought to be sterile, were observed in T. clavigera at sites near marinas and harbours. The effects of organotin pollution on the population of T. clavigera were observed at the sites near marinas. From the results of the additional country-wide survey on imposex in T. clavigera during 1993-1995, serious organotin pollution and imposex symptoms in the species are still continuing in Japan. © 1997 by John Wiley & Sons, Ltd.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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