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  • 1
    Electronic Resource
    Electronic Resource
    Juvenile hormone catabolism inManduca sexta: Homologue selectivity of catabolism and identification of a diol-phosphate conjugate as a major end product (1993)
    Springer
    Cellular and molecular life sciences 49 (1993), S. 988-994 
    Details
    ISSN: 1420-9071
    Keywords: Juvenile hormone esterase ; juvenile hormone epoxide hydrolase ; juvenile hormone binding protein ; reversed-phase liquid chromatography assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We studied time-dependent metabolism of (10R)-[3H] juvenile hormone (JH) III and (10R, 11S)-[3H]JH I injected intoManduca sexta larvae; the hormones are metabolized to polar metabolites, expecially the JH acid-diol, and an unknown. Products were analyzed using a reversed-phase liquid chromatography assay. (10R)-JH III is metabolized much more rapidly than (10R, 11S)-[3H]JH I, whether injected seperately or as a mixture of hormones. The unknown metabolites of JH I and JH III were identified as phosphate conjugates of JH I and JH III diol by tandem mass spectral analysis of isolated samples. The phosphate conjugate of JH I diol is the principle end product of JH I metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02125646
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  • 2
    Electronic Resource
    Electronic Resource
    Titers of ecdysone, 20-hydroxyecdysone and juvenile hormone III throughout the life cycle of a hemimetabolous insect, the ovoviviparous cockroachNauphoeta cinerea (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 913-917 
    Details
    ISSN: 1420-9071
    Keywords: Ecdysteroids ; juvenile hormone III ; developmental changes ; cockroach ; Nauphoeta cinerea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Titers of ecdysone, 20-hydroxyecdysone and juvenile hormone III were measured in whole body extracts or hemolymph of embryos, first, penultimate and last stadium nymphs, and adult females ofNaupoheta cinerea. We used a gas-chromatography/mass spectrometry method for quantifying juvenile hormone and a radio-immunoassay for ecdysteroid determination. Juvenile hormone III is particularly abundant in the embryonic stage (up to 960 ng/g), at a low level in first and penultimate stadium nymphs (2–10 ng/ml) and almost absent in the last nymphal stadium; in the adult female the juvenile hormone titer rises to 180 ng/ml in hemolymph during rapid oocyte growth. The titers of ecdysone and 20-hydroxyecdysone undergo similar fluctuations in the embryonic and nymphal stages, being highest at the time of cuticle formation in the embryo and a few days before the nymphal and adult molts (around 100–200 ng/ml for exdysone and 2–4 μg/ml for 20-hydroxyecdysone).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01970010
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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