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  • Karyotype  (1)
  • Key words Evolutionary gene transfer  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The gene for mitochondrial ribosomal protein S14 has been transferred to the nucleus in Arabidopsis thaliana (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 139-144 
    Details
    ISSN: 1617-4623
    Keywords: Key words Evolutionary gene transfer ; Plant mitochondria ; Ribosomal protein S14 ; Mitochondrial targeting peptide ; Nuclear intron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The transfer of genetic information from the mitochondrion to the nucleus is thought to be still underway in higher plants. The mitochondrial genome of Arabidopsis thaliana contains only one rps14 pseudogene. In this paper we show that the functional gene encoding mitochondrial ribosomal protein S14 has been translocated to the nucleus. This gene transfer is a recent evolutionary event, which occurred within Cruciferae, probably after the divergence of Arabidopsis and Brassica napus. A 5′ extension of the rps14 reading frame encodes a presequence which, in vitro, targets the polypeptide to isolated mitochondria and is cleaved off during or after import. No intron was found at the junction of the targeting presequence with the mitochondrially derived sequence, which are directly connected. By contrast, a 90-bp intron, which is removed by splicing to give a mature poly(A)+mRNA of 0.9 kb, is located in the 3′ non-coding region. To our knowledge, this is the first report of an intron in such a position in a functional transferred gene in higher plants, and suggests that exon shuffling may have been involved in the acquisition of elements necessary for expression in the nucleus. Putative roles of this intron in polyadenylation and enhancement of gene expression are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051068
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic diversity and vegetative compatibility among Trichoderma harzianum isolates (1997)
    Springer
    Molecular genetics and genomics 256 (1997), S. 127-135 
    Details
    ISSN: 1617-4623
    Keywords: Key words Trichoderma ; Karyotype ; Vegetative compatibility ; Molecular variation ; Systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Trichoderma harzianum is the collective name of a set of asexual fungal strains which exhibit heterogeneity in genome structure, DNA sequence and behavior. Contour-clamped homogeneous field (CHEF) electrophoresis of the chromosomes of ten isolates of T. harzianum revealed six clearly distinct electrophoretic karyotypes. Of the ten isolates analyzed, four (GH12, G109, Y and YF) could be classified in a single group with identical karyotypes, while the strains T35 and 315 formed a second group. The genome size characteristic of the different isolates fell into a broad range varying from 29.6 to 56.1 Mb. Gene assignments to the resolved chromosomes showed that all genes analyzed were localized on equivalent chromosomes in the isolates belonging to the same group. Analysis of randomly amplified polymorphic DNAs from the ten isolates confirmed the classification into groups and allowed us to distinguish between isolates T35 and 315, as well as between isolates GH12, G109, Y and YF. Direct confrontation assays using isolates of the same group showed compatible interactions, whereas the same experiment carried out with isolates of different groups showed an incompatible interaction characterized by an area of cell damage. Microscopic observation of the compatible interactions showed hyphal fusions between the isolates, similar to those described for vegetative compatible groups in other fungi. The molecular karyotypes correlated well with the compatibility of the isolates. In addition, we have evaluated both electrophoretic karyotype and randomly amplified polymorphic DNAs analysis as criteria for grouping isolates within the genus according to their capacity for biocontrol of plant pathogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050554
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    Paper (German National Licenses)
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