ISSN:
1573-9368
Keywords:
transgenic mice
;
screening
;
PCR analysis
;
epithelial cell lysates
;
Kawasaki buffer
;
rectum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Conventional screening protocols for transgene integration in mice employ tail tips or blood samples as sources to obtain genomic DNA preparations. We have developed a simple alternative non-surgical method. Epithelial cells are scraped off the inner surface of the rectum with a sterile plastic inoculation loop and are lysed with Kawasaki buffer. The lysate can be directly examined in a polymerase chain reaction (PCR) analysis without any need for further DNA purification. This procedure causes minimal harm and stress to the animals and repeated samples can be obtained as often as necessary. This technique has been used successfully to identify transgenic mice from a number of different lines. The method allows quick screening of numerous animals and contributes to a reduction of the number of surgical biopsies required
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008824509988
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