ISSN:
1438-8359
Keywords:
Ketamine
;
Muscarinic acetylcholine receptors
;
Forebrain
;
Up-regulation
;
Behavior sensitivity to scopolamine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract To study the effects of repeated ketamine administration (0: saline, 12.5, 25, and 50 mg·kg−1 every 3 days for a total of five times, subcutaneously) on the central muscarinic acetylcholine receptors (mAchRs), receptor binding assays of mAchR were carried out in the forebrain of mice, using [3H]quinuclidinyl benzilate ([3H]QNB) as a ligand. We also examined whether repeated ketamine administration could modify the sensitivity to scopolamine (0.5 mg·kg−1) (a muscarinic antagonist). Repeated ketamine administration produced a significant increase in the receptor density values (Bmax) for [3H]QNB (1520±51 fmol·mg protein−1 for the control group, 1650±43 for the 12.5 mg·kg−1 group, 1966±70 for the 25 mg·kg−1 group and 2064±125 for the 50 mg·kg−1 group) (P〈0.05, when the 25 mg·kg−1 and 50 mg·kg−1 groups were compared to the control group) without any change in apparent affinity. Repeated ketamine reduced scopolamine-induced hyperlocomotion at 50 mg·kg−1 (P〈0.05). We conclude that repeated ketamine administration produces up-regulation of mAchRs, which is probably associated with the altered Ach transmission of the central nervous system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02479877
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