ISSN:
1432-2048
Keywords:
Key words: Adhesion
;
Diatom
;
Extracellular matrix
;
Gliding (diatom)
;
Proteoglycan
;
Stauroneis (extra cellular proteoglycans)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. Diatoms are unicellular microalgae encased in a siliceous cell wall, or frustule. Pennate diatoms, which possess bilateral symmetry, attach to the substratum at a slit in the frustule called the raphe. These diatoms not only adhere, but glide across surfaces whilst maintaining their attachment, secreting a sticky mucilage that forms a trail behind the gliding cells. We have raised monoclonal antibodies to the major cell surface proteoglycans of the marine raphid diatom Stauroneis decipiens Hustedt. The antibody StF.H4 binds to the cell surface, in the raphe and to adhesive trails and inhibits the ability of living diatoms to adhere to the substratum and to glide. Moreover, StF.H4 binds to a periodate-insensitive epitope on four frustule-associated proteoglycans (relative molecular masses 87, 112, and 〉200 kDa). Another monoclonal antibody, StF.D5, binds to a carbohydrate epitope on the same set of proteoglycans, although the antibody binds only to the outer surface of the frustule and does not inhibit cell motility and adhesion.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004250050184
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