ISSN:
1432-1432
Keywords:
Key words: Microsporidians —Glugea plecoglossi— Eukaryotes — Mitochondrion-lacking protozoa — Elongation factor 1α— Protein phylogeny
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. Partial regions of the mRNA encoding a major part of translation elongation factor 1α (EF-1α) from a mitochondrion-lacking protozoan, Glugea plecoglossi, that belongs to microsporidians, were amplified by polymerase chain reaction (PCR) and their primary structures were analyzed. The deduced amino acid sequence was highly divergent from typical EF-1α's of eukaryotes, although it clearly showed a eukaryotic feature when aligned with homologs of the three primary kingdoms. Maximum likelihood (ML) analyses on the basis of six different stochastic models of amino acid substitutions and a maximum parsimony (MP) analysis consistently suggest that among eukaryotic species being analyzed, G. plecoglossi is likely to represent the earliest offshoot of eukaryotes. Microsporidians might be the extremely ancient eukaryotes which have diverged before an occurrence of mitochondrial symbiosis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02198852
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