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Digitale Medien

Transgenic oat plants via visual selection of cells expressing green fluorescent protein (2000)

Kaeppler, H. F. ; Menon, G. K. ; Skadsen, R. W. ; [weitere]

Springer

Plant cell reports 19 (2000), S. 661-666

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Details

ISSN: 1432-203X

Schlagwort(e): Key words Genetic engineering ; Green fluorescent protein ; Oat ; Transformation

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract New selectable markers and selection systems are needed to increase the efficiency and flexibility of plant transformation. The objective of this research was to determine if the green fluorescent protein (gfp) gene could be utilized as a visual selectable marker for transformation of oat (Avena sativa L.). A modified gfp gene was delivered into oat cells by microprojectile bombardment. Cell clusters expressing gfp were visually identified using fluorescence microscopy and physically isolated at each subculture. Eleven independent transgenic cell lines were obtained, and fertile plants regenerated from all lines. Transgene integration and expression were confirmed in transgenic plants and progeny. Transgene expression segregated in a 3 : 1 ratio in progeny of the majority of the transgenic lines.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s002999900167

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Digitale Medien

Low-temperature polymerization of isobutyl vinyl ether (1966)

Blake, G. J. ; Carlson, A. M.

New York : Wiley-Blackwell

Journal of Polymer Science Part A-1: Polymer Chemistry 4 (1966), S. 1813-1820

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Details

ISSN: 0449-296X

Schlagwort(e): Physics ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: Isobutyl vinyl ether has been polymerized under conditions well known to yield isotactic polymer, viz., with boron trifluoride etherate at 78°C. in a nonpolar hydrocarbon diluent. A particular mixed solvent ratio and previous dissolution of catalyst enabled the polymerization to proceed homogeneously at the beginning. By following the temperature rise in an initially thermostatted system, we showed that the progress of the reaction eventually proceeded via a homogeneous phase to a gellike phase. Isotactic polymer is shown to be produced in both steps by a mechanism of slow chain propagation.

Zusätzliches Material: 3 Ill.