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  • Key words Maize pollen  (1)
  • Key words Sorghum bicolor  (1)
  • 1
    Digitale Medien
    Digitale Medien
    gaMS-1: a gametophytic male sterile mutant in maize (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 216-220 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Key words Maize pollen ; Male sterility ; Microsporogenesis ; Gametophytic gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Several pollen-specific genes from different species have been isolated and characterized at the molecular level, but the precise role of most of them is unknown. Mutant analysis represents a direct approach to uncovering gene function, but the paucity of available mutants affecting pollen development and/or function and the poor characterization of the known mutants have so far limited the exploitation of this approach. Here we present the cytological characterization of gametophytic male sterile-1 (gaMS-1), a maize mutant that we identified in a program of transposon insertion mutagenesis for the production of mutations in gametophytically acting genes involved in microsporogenesis. gaMS-1 is expressed during or immediately after the first microspore division and leads to the production of immature, non-functional pollen grains. The mutation appears to affect the events leading to the developmental switch that follows the first microspore mitosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004970050034
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Characterization and mapping of simple sequence repeats (SSRs) in Sorghum bicolor (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 66-72 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Sorghum bicolor ; Simple sequence repeats (SSRs) ; Polymorphism ; Mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A total of 13 SSR loci were characterized in Sorghum bicolor. Ten of these loci were isolated by screening sorghum genomic AG-enriched libraries with labelled poly(AG)/poly(CT), the other three were derived from database searches. In order to explore the degree of polymorphism detectable in this species by this type of molecular marker, the SSR markers were tested on nine inbred lines of S. bicolor of different geographic origin. PCR analysis on acrylamide gels revealed a high degree of polymorphism (δT=0.80). One locus, in particular, allowed the identification of all of the nine inbred lines used in our study. Seven of these SSR markers were mapped, using an existing sorghum RFLP map.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050533
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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