ISSN:
1432-2013
Keywords:
Key words Pancreas
;
Islet cells
;
Cell volume
;
Na+-2Cl
;
-K+ cotransporter
;
Na+-H+ exchange
;
Cl
;
-HCO3
;
exchange
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract This study investigated regulatory volume increase (RVI) in rat pancreatic β-cells. Volume changes in isolated β-cells were measured by a video-imaging method. Cell shrinkage was induced by exposure to solutions made hypertonic by the addition of 100 mM mannitol. In HEPES-buffered solutions, β-cells exhibited an RVI which was almost completely abolished by 10 μM bumetanide. These data indicate that Na+-2Cl–-K+ cotransporters make a major contribution to RVI in β-cells. In HCO3 –-buffered solutions, however, an RVI was observed in the presence of 10 μM bumetanide. This bumetanide-insensitive component of RVI was inhibited by 100 μM amiloride or 100 μM 4,4’-diisothiocyanatostilbene-2,2’-disulphonic acid (DIDS). These data suggest that, in addition to the Na+-2Cl–-K+ cotransporter, functionally coupled Na+-H+ exchangers and Cl–-HCO3 – exchangers may also contribute to RVI in pancreatic β-cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004240050505
Permalink
