ISSN:
1617-4623
Keywords:
Key words Schizophyllum commune
;
Mating type
;
Homeodomain
;
Two-hybrid system
;
Protein interaction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The Aα mating locus of the woodrotting fungus Schizophyllum commune encodes two multiallelic genes, Y and Z, which regulate the A-pathway of development. The Y alleles contain a homeobox, suggesting that the Y proteins may be DNA-binding regulatory proteins. During mating, development is induced when Y from one mating partner interacts with Z from the other mating partner; self combinations of Y and Z are inactive. Two-hybrid analyses indicate that nonself combinations of Y and Z form heteromultimers and self combinations do not. To understand Y-Z binding and self- nonself recognition further we used mutagenesis and chimeras to identify regions in one allele of Z (Z5) that are involved in these processes. Here we report the results, which broadly define regions in Z5 that are essential for activity, Y-Z binding and Z5 allelic specificity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02173981
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