Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Detection of genetically modified insect-resistant Bt maize by means of polymerase chain reaction (1997)
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 205 (1997), S. 442-445 
    Details
    ISSN: 1431-4630
    Keywords: Key words Polymerase chain reaction ; Genetically modified maize ; Bacillus thuringiensis ; Insect-resistant maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A method for the detection of genetically modified insect-resistant maize expressing a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis is described. The procedure includes: (1) extraction of genomic DNA, (2) amplification of the inserted synthetic gene by means of the polymerase chain reaction (PCR) method, and (3) characterization and confirmation of the PCR product by Southern hybridization with a digoxigenin-labelled oligonucleotide probe and DNA sequencing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050196
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Detection of the genetic modification in heat-treated products of Bt maize by polymerase chain reaction (1998)
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 206 (1998), S. 203-207 
    Details
    ISSN: 1431-4630
    Keywords: Key words Polymerase chain reaction ; Genetically modified maize ; Bacillus thuringiensis ; Thermal treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A method for the detection of the genetic modification in thermally treated products from insect-resistant maize expressing a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis is described. The probability of detection of the transgene in thermally treated products was increased by choosing a short (211 bp) polymerase chain reaction (PCR) amplicon; the specificity was ensured by including a promoter region adjacent to the cryIA(b) gene in the target sequence. The influence of pH during thermal treatment of maize on the detectability of the transgene is demonstrated using polenta as a model for products made from this cereal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050243
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...