ISSN:
0021-9304
Keywords:
transforming growth factor-beta1 (TGF-β1)
;
wax-like polymer
;
biodegradation
;
biocompatibility
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
The study reported describes an experimental biodegradable polymer ceramic composite with wax-like handling properties that was combined with 2.0 μg of recombinant human transforming growth factor beta (rhTGF-β1). The polymer/rhTGF-β1 combination was introduced into standard-sized calvarial defects in rabbits to evaluate biodegradability, biocompatibility, hemostasis control, and bone promotion. The experimental wound model was a standard-sized circular calvarial defect 8 mm in diameter. The experimental design included 24 skeletally mature New Zealand white rabbits divided evenly between two time periods (6 and 12 weeks) and among three experimental treatments (untreated defects and defects treated with polymer with or without rhTGF-β1). Evaluations consisted of clinical examinations, standardized radiography, radiomorphometry, as well as histology and histomorphometry. Data were analyzed by an Analysis of Variance (ANOVA) and Fisher's Protected Least Significant Difference test at each time period (level of significance p≤ 0.05). Radiomorphometry data indicated that standard-sized defects treated with the wax-like polymer alone and the polymer plus 2.0 μg of TGF-β1 were significantly more radiopaque than control sites at both 6 and 12 weeks. Histomorphometric data revealed the amount of new bone was significantly greater at 6 weeks in the polymer plus 2.0 μg of TGF-β1 and in the control group than in the polymer alone. Moreover, at 12 weeks, there was significantly more new bone in the control than in either the polymer alone or the polymer plus 2.0 μg of TGF-β1. We speculate the incomplete biodegradation of the polymer ceramic composite contributed to the radiopacity and may have retarded osseous regeneration. It is important that the bone wax-like polymer material was biocompatible and acted as a hemostatic agent. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 41, 584-592, 1998.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
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