ISSN:
1432-203X
Keywords:
Key words Zygote
;
Egg cell
;
Plant regeneration
;
Individual culture
;
Rice
;
Oryza sativa
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A simple mechanical method has been developed which allows the routine isolation of unfertilized and fertilized egg cells from ovules of Japonica and Indica rice varieties. In the experiments described, the majority of the egg cells and zygotes survived the isolation procedure when the donor plants were in a vigorous state. About 40% of the surviving zygotes underwent sustained development when cultured in Millicell inserts with a non-morphogenic rice feeder-cell culture. Nearly all zygote-derived callus cultures regenerated multiple shoots, which could be subsequently rooted with high efficiency. Zygote-derived plantlets matured to fertile plants when transplanted to soil. So far, about 80 independent plants each from the Japonica variety 'Taipei309' and the Indica variety 'IR58' have been regenerated. The potential of this single-cell regeneration system for marker gene-free transformation is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050722
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