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  • 1
    Electronic Resource
    Electronic Resource
    Genetic variability in callus formation and regeneration of garlic (Allium sativum L.) (1999)
    Springer
    Plant cell reports 18 (1999), S. 434-437 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAllium sativum ; Callus induction ; Organogenesis ; Genetic variability ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty garlic Allium sativum (L.) genotypes were analysed for genetic variation in their ability to form callus (in one medium) and regenerate shoots (in four different media). Genotypes showed significant differences in the analysis of variance of all the traits tested. The accession Printanor showed the best general behaviour, with 83% callus-producing explants, 44.7% organogenic explants, and 15.35 shoots/g of callus. The best regeneration medium was MBO, without growth regulators. Shoot production capacity was examined with the additive main effects and multiplicative interaction model that proved to be a powerful tool for analysis and easy comprehension of the strong genotype×medium interactions frequently observed in in vitro culture systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050599
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L. (1998)
    Springer
    Plant cell reports 17 (1998), S. 737-741 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAllium sativum ; Transformation ; Particle bombardment ; Nuclease ; Aurintricarboxylic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biolistic particle delivery system was used to transfer the uidA gene into different garlic tissues, including regenerable calli. These tissues showed a high endogenous nuclease activity preventing exogenous DNA expression. After submerging the tissues for 24 h in a 2 mm solution of the nuclease inhibitor aurintricarboxylic acid, transient expression of the uidA gene was detected. Furthermore, vacuum infiltration of aurintricarboxylic acid into the garlic tissues significantly increased the percentage of explants showing transient expression. Among four plasmids tested, pDE4 containing the CaMV35S-uidA construct produced the best results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050475
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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