Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Life and Medical Sciences  (2)
  • Key wordsEscherichia coli  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A mutational study of the ArcA-P binding sequences in the aldA promoter of Escherichia coli (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 170-176 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsEscherichia coli ; Arc two-component system ; Signal transduction ; Aldehyde dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aldA gene (encoding aldehyde dehydrogenase) of Escherichia coli is anaerobically repressed by ArcA-P, the phosphorylated response regulator of the ArcB/A two-component signal transduction system. The promoter region of aldA contains two 10-bp sequences (5′-TGTTAATTAA-3′) that perfectly match the proposed ArcA-P binding consensus (5′-[A/T]GTTAATTA[A/T]-3′). One consensus sequence is on the coding strand (−13 to −4 from the transcriptional start point), whereas the other is on the template strand (position −2 to −11). In this study we used the aldA promoter to test the validity of the proposed consensus sequence. DNase I protection experiments confirmed the 10-bp sequence to be a strong ArcA-P binding site. Alteration of the wild-type sequence from 5′-TGTTAATTAAC-3′ to 5′-TCTTAATTAAG-3′ or 5′-TATTAATTAAT-3′ by site-directed mutagenesis markedly decreased the in vitro affinity of the promoter region for ArcA-P, and abolished the anaerobic repression of mutant attλ::Φ (aldA′-lacZ) transcriptional reporter constructs. Both the in vitro and in vivo results therefore support the proposed consensus sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050954
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Glycerol transport and phosphorylation by rat hepatocytes (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 117 (1983), S. 230-234 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The entry of glycerol into isolated rat hepatocytes appears to be catalyzed by a specific carrier. At a physiological concentration of 0.1 mM, glycerol utilization is rate limited by the permeation step. Intracellular glycerol is trapped by an excess of glycerol kinase, which has a higher apparent affinity for the substrate than that of the membrane carrier. The entry of glycerol into the hepatocytes is highly sensitive to inhibition by monoacetin and cytochalasin B, but not by DL-1,2-propanediol, erythritol, D-glucose, D-galactose, D-mannose, or D-fructose.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041170214
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The selection of a stable rat hepatoma variant with concomitant increase in ploidy and permeability to glycerol (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 94 (1978), S. 197-203 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: By repeated selection for longer survival in an isotonic solution of glycerol, a stable subline of Novikoff rat hepatoma cells has been isolated. The cells exhibit markedly increased resistances to osmotic lysis in isotonic solutions of glycerl. They are twice as large and have twice as many chromosomes as cells of the parental line. It is suggested that the osmotic stress procedure can be extended for the selection of numerous kinds of mutants and can be used as a method of analysis of membrane properties.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040940209
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...