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  • 1
    Electronic Resource
    Electronic Resource
    A mutational study of the ArcA-P binding sequences in the aldA promoter of Escherichia coli (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 170-176 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsEscherichia coli ; Arc two-component system ; Signal transduction ; Aldehyde dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aldA gene (encoding aldehyde dehydrogenase) of Escherichia coli is anaerobically repressed by ArcA-P, the phosphorylated response regulator of the ArcB/A two-component signal transduction system. The promoter region of aldA contains two 10-bp sequences (5′-TGTTAATTAA-3′) that perfectly match the proposed ArcA-P binding consensus (5′-[A/T]GTTAATTA[A/T]-3′). One consensus sequence is on the coding strand (−13 to −4 from the transcriptional start point), whereas the other is on the template strand (position −2 to −11). In this study we used the aldA promoter to test the validity of the proposed consensus sequence. DNase I protection experiments confirmed the 10-bp sequence to be a strong ArcA-P binding site. Alteration of the wild-type sequence from 5′-TGTTAATTAAC-3′ to 5′-TCTTAATTAAG-3′ or 5′-TATTAATTAAT-3′ by site-directed mutagenesis markedly decreased the in vitro affinity of the promoter region for ArcA-P, and abolished the anaerobic repression of mutant attλ::Φ (aldA′-lacZ) transcriptional reporter constructs. Both the in vitro and in vivo results therefore support the proposed consensus sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050954
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  • 2
    Electronic Resource
    Electronic Resource
    The requirement of ArcA and Fnr for peak expression of the cyd operon in Escherichia coli under microaerobic conditions (1991)
    Springer
    Molecular genetics and genomics 226 (1991), S. 209-213 
    Details
    ISSN: 1617-4623
    Keywords: Microaerobiosis ; cyd ; arcA ; fnr ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Transcriptional regulation of cyd, encoding the cytochrome d complex for O2 scavenging, was studied in Escherichia coli by monitoring ϕ(cyd-lac) expression under atmospheres containing 0–21% O2. Peak expression of this operon occurred under microaerobic conditions. Mutations in arcA (a trans-acting regulatory gene involved in aerobic respiration) greatly lowered ϕ(cyd-lac) expression under all conditions. Mutations in fnr (a trans-acting regulatory gene involved in anaerobic respiration) shifted the pattern of ϕ(cyd-lac) expression, lowering the microaerobic and aerobic expression. In an arcA fnr double mutant, ϕ(cyd-lac) expression became insignificant, irrespective of the availability of O2. It thus appears that the expression of the ϕ(cyd-lac) operon is under dual control by the two pleiotropic activators, ArcA and Fnr, which interact to give the peak microaerobic expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273605
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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