ISSN:
1432-072X
Keywords:
Key wordsEscherichia coli
;
Stringent response
;
Amino acid transport
;
Glutamate excretion
;
relA
;
spoT
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Glutamate excretion due to amino acid starvation was investigated in "stringent" and "relaxed" strains of Escherichia coli. The observed excretion process is relA-dependent, carrier-mediated, and glutamate-specific. After induction, excretion was detected within less than 2 min and continued for more than 5 h with a rate of 7–10 nmol (mg dry weight)–1 min–1. Using carbonyl cyanide m-chlorophenylhydrazone or polymyxin B nonapeptide, together with valinomycin, it was shown that glutamate excretion is driven by the membrane potential.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050231
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