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  • Keywords: Bending and piercing; Knowledged-base rules; Lead frame  (1)
  • top2ts  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    The international journal of advanced manufacturing technology 16 (2000), S. 624-634 
    ISSN: 1433-3015
    Keywords: Keywords: Bending and piercing; Knowledged-base rules; Lead frame
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper describes the development of computer-aided design of irregular-shaped sheet metal products and 32LD PLCC lead frames and semiconductors, requiring very precise piercing operations for progressive working. The approach to the CAD system is based on knowledge-based rules. Knowledge for the CAD system is formulated from plasticity theories, experimental results, and the empirical knowledge of field experts. This system has been written in AutoLISP using AutoCAD on a personal computer and the I-DEAS drafting programming language on the I-DEAS master series drafting with an HP 9000/715(64) workstation. Transference of data between Auto-CAD and I-DEAS master series drafting is accomplished using DXF and IGES methods. This system is composed of five main modules, which are input and shape treatment, production feasibility check, strip-layout, data-conversion, and die-layout modules. Based on knowledge-based rules, the system is designed by considering several factors, such as V-notches, dimple, pad chamfer, spank, cavity punch, camber, coined area, cross bow, material and thickness of product, complexities of blank geometry and punch profile, and availability of presses. As forming processes and the die design system using 2D geometry recognition are integrated with the technology of process planning, die design, and CAE analysis, the standardisation of die parts for lead frames requiring a high precision piercing process is possible. The die-layout drawing generated by the die-layout module is displayed in graphic form. Results obtained for each module provide help to the designer and the manufacturer of lead frames for semiconductors.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Key words Rat ; DNA topoisomerase IIα ; Saccharomyces cerevisiae ; top2ts ; Complementation ; Leucine zipper
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A series of yeast expression plasmids which comprise segments of the cDNA sequences encoding rat topo IIα have been constructed. The transcription of these constructs is under the control of the yeast GAL1 promoter. Galactose-dependent expression of the cloned rat topo IIα cDNA complemented a yeast top2 ts mutation, as well as a deletion mutation at the yeast TOP2 locus. Truncation of 12 N-terminal amino acids and/or 158 C-terminal amino acids of rat topo IIα had no effect on its ability functionally to substitute for top2 ts . Moreover, a cDNA construct with mutated putative leucine zipper domain (amino acids 993–1013) retained the complementation activity. These observations suggest that transformants capable of conditional topo IIα expression can be exploited as a useful model system for studies on the structure-function relationships of wild-type and mutated topo IIα, as well as the interplay of potential antitumor drugs with the enzyme.
    Type of Medium: Electronic Resource
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