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  • 1
    Electronic Resource
    Electronic Resource
    Sequence and expression of the mRNA encoding the chymotrypsin inhibitor in winged bean (Psophocarpus tetragonolobus (L.) DC.) (1989)
    Springer
    Plant molecular biology 12 (1989), S. 51-58 
    Details
    ISSN: 1573-5028
    Keywords: cDNA sequence ; expression library ; in vitro translation ; Kunitz-type proteinase inhibitor ; mRNA accumulation in vivo ; signal peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The accumulation of the Kunitz-type chymotrypsin inhibitor WCI-3 in winged bean seeds is controlled developmentally. In vitro translation experiments showed that the WCI-3 mRNA was present in 35- and 40-day-old immature seeds after flowering. The size of the in vitro translation product is about 2 000 Da larger than that of the mature WCI-3 protein. The WCI-3 cDNA clones were isolated from a λgtll cDNA library of 35-day-old immature seeds by immunoscreening. A nearly full-length cDNA clone was obtained containing an open reading frame of 207 amino acid residues. The deduced sequence of the 183 carboxy terminal amino acids coincides precisely with the amino acid sequence determined for purified WCI-3. The amino terminal extension of 24 residues has the characteristics of a signal peptide. Northern hybridization analysis of total poly(A)+ RNA showed that the WCI-3 mRNA is approximately 900 nucleotides long and accumulates in 35- and 40-day-old but not in 30-day-old immature seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017447
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of tobacco mosaic virus RNA in transgenic plants (1988)
    Springer
    Molecular genetics and genomics 211 (1988), S. 520-525 
    Details
    ISSN: 1617-4623
    Keywords: Tobacco mosaic virus ; Transgenic plant ; In planta expression ; Ti plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tobacco mosaic virus (TMV) is a message-sense, single-stranded RNA virus that infects many Solanaceae plants. A full-length cDNA copy of TMV genomic RNA was constructed and introduced into the genomic DNA of tobacco plants using a disarmed Ti plasmid vector. Transformed plants showed typical symptoms of TMV infection, and their leaves contained infectious TMV particles. This is the first example of the expression of RNA virus genomic RNAs in planta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425710
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cross protection in transgenic tobacco plants expressing a mild strain of tobacco mosaic virus (1988)
    Springer
    Molecular genetics and genomics 215 (1988), S. 173-175 
    Details
    ISSN: 1617-4623
    Keywords: Tobacco mosaic virus ; Mild strain ; Cross protection ; Transgenic plant ; Ti plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cross protection of plant viruses is a phenomenon in which plants infected with one strain of a virus are protected from the effects of superinfection by other related strains. Recently, we have succeeded in the introduction and expression of a cDNA copy of the tobacco mosaic virus (TMV) genomic RNA in transgenic tobacco plants. Using this system, we introduced a cDNA copy of a mild strain of TMV into tobacco plants. The transgenic plants did not develop any severe symptoms upon inoculation with a virulent TMV strain, indicating that these transgenic plants were cross protected against TMV infection. The system described here can be a useful model system to study the mechanism(s) of cross protection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331321
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    Paper (German National Licenses)
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