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  • 1
    Electronic Resource
    Electronic Resource
    Two-compartment behavior during transport of folate compounds in L1210 cell plasma membrane vesicles (1982)
    Springer
    The journal of membrane biology 68 (1982), S. 19-28 
    Details
    ISSN: 1432-1424
    Keywords: folate transport ; plasma membrane vesicles ; L1210 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The transport of [3H] 1,l 5-formyltetrahydrofolate, [3H] folic acid, and [3H]methotrexate by L1210 cell plasma membrane vesicles exhibited multicompartmental behavior. Two separate vesicular compartments (parallel relationship) of approximately equal volume were revealed during measurements of influx and efflux. Flux in one compartment was rapid, saturable, highly temperature-sensitive, and inhibited by pCMBS. Flux in the other compartment exhibited all of the characteristics of passive diffusion. These results imply that our plasma membrane vesicle preparations consist of a mixture of two functional species. Transport of folate into one of these species occurs by passive diffusion alone, whereas transport into the other kind of vesicle occurs by both passive diffusion and carrier-facilitated transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872250
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of metabolic deprivation on methotrexate transport in L1210 leukemia cells: Further evidence for separate influx and efflux systems with different energetic requirements (1984)
    Springer
    The journal of membrane biology 78 (1984), S. 9-17 
    Details
    ISSN: 1432-1424
    Keywords: methotrexate ; transport ; L1210 cells ; nonidentical influx ; efflux routes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Measurements of methotrexate transport in L1210 cells in the presence and absence ofd-glucose reveal that both influx and efflux are depressed in the absence ofd-glucose, whereas the steady-state accumulation of drug is enhanced. The reason for the increase in steady state is that the relative decline in efflux is greater than the decline in influx. Analysis of the concentration dependence of steady-state methotrexate accumulation ind-glucose-deprived cells indicates a linear relationship consistent with a one-carrier active transport model. Similar data in nondeprived cells is highly nonlinear and strongly supports the postulate that under physiological conditions influx and efflux of methotrexate are mediated by separate carrier systems. These results indicate that the efflux system, preferentially transporting methotrexate under normal conditions, cannot operate in the absence ofd-glucose, whereas the influx system is only partially inhibited under conditions of glucose deprivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872527
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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