ISSN:
1612-1112
Keywords:
Open-tubular liquid chromatography
;
External peak broadening
;
Exponentially modified Gaussian model
;
Mass Spectrometry
;
Laser-induced fluorescence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary To optimize performance in Open-Tubular Liquid Chromatography (OTLC) it is necessary to minimize external peak broadening. To see how to reduce the external broadening an insight into its origins is required. This can be obtained by careful evaluation of experimental results with peak deconvolution methods based on the exponentially modified Gaussian model and comparison with theoretical predictions. It is assumed that the column response is Gaussian and that the responses due to the external effects are exponential. For peak deconvolution the algorithm described by Yau was used. Simulations were carried out to check the performance of the algorithm in calculating the standard deviation and the time constant. The effects of the presence of more than one time constant and of the number of data points and their position were investigated. The limits within which reliable results can be obtained are reported. Experimental results were obtained with laser-induced fluorescence and mass spectrometric detection. It is shown that the Yau algorithm can be used to obtain physically realistic estimates of the contribution to peak distortion in the various system components. By suitable design external effects can be reduced to the order of 1 nl and in some cases even lower limits can be reached.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02269062
Permalink
