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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 31 (2000), S. 379-384 
    ISSN: 1432-0789
    Keywords: Key words Bioassay ; Fertile islands ; Latrine ; Mediterranean ecosystem ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Degradation of land in vulnerable areas can be significantly reduced by the maintenance or establishment of plant cover and diversity. Animals can facilitate plant diversity by grazing, by dispersing seeds or by contributing, through excreta, to the heterogeneous distribution of nutrients in soil. We investigated the latter property by examining the effects of rabbit (Oryctolagus cuniculus L.) dung deposition on soil properties in three adjacent plant communities at a semi-arid site in south-east Spain. Rabbit faecal pellets had concentrations of total N and P comparable to dung of stock animals, with K and Mg somewhat lower, although decomposition rates at this site are evidently very low. There was no significant difference in pH, but conductivity and concentrations of organic C, N (as NH4 + and NO3 –), K, P and Mg in soils from rabbit latrines were significantly greater relative to controls in each community. Barley plants grown as a bioassay of soil fertility had significantly greater total biomass, and lower root : shoot ratios in latrine compared to control soils. There were differences among communities in conductivity and concentrations of N, P and organic C under latrines which were reflected in the bioassay. Although latrines only comprised approximately 0.1% of the ground surface area in each community, they make significant localised contributions to soil fertility and may therefore be important in establishing and maintaining plant cover.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Cytokinin ; Mutant (barley) ; Pisum ; Potassium and stomata protoplast ; Stomata (epidermis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Epidermis is easily detached from both adaxial and abaxial surfaces of leaf four of the Argenteum mutant of Pisum sativum L. The isolated epidermis has stomata with large, easily-measured pores. Hairs and glands are absent. The density of stomata is high and contamination by mesophyll cells is low. In the light and in CO2-free air, stomata in isolated adaxial epidermis of Argenteum mutant opened maximally after 4 h incubation at 25°C. The response of stomata to light was dependent on the concentration of KCl in the incubation medium and was maximal at 50 mol m-3 KCl. Stomata did not respond to exogenous kinetin, but apertures were reduced by incubation of epidermis on solutions containing between 10-5 and 10-1 mol m-3 abscisic acid (ABA). The responses of stomata of Argenteum mutant to light, exogenous KCl, ABA and kinetin were comparable with those described previously for stomata in isolated epidermis of Commelina communis. A method for preparing viable protoplasts of guard cells from isolated epidermis of Argenteum mutant is described. The response of guard cell protoplasts to light, exogenous KCl, ABA and kinetin were similar to those of stomata in isolated epidermis except that the increase in volume of the protoplasts in response to light was maximal at a lower concentration of KCl (10 mol m-3) and that protoplasts responded more rapidly to light than stomata in isolated epidermis. The protoplasts did not respond to exogenous kinetin, but when incubated for 1 h in the light and in CO2-free air on a solution containing 10-3 mol m-3 ABA, they decreased in volume by 30%. The advantages of using epidermis from Argenteum mutant for experiments on stomatal movements are discussed.
    Type of Medium: Electronic Resource
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