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  • Life and Medical Sciences  (1)
  • Maize alcohol dehydrogenase  (1)
  • chromosome mapping  (1)
  • 1
    ISSN: 1573-5028
    Keywords: alcohol dehydrogenase ; anaerobiosis ; chromosome mapping ; gene expression ; Petunia hybrida ; plant development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genomic clone for an alcohol dehydrogenase (Adh) gene has been isolated fromPetunia hybrida cv. V30 by screening aPetunia genomic library with a maizeAdh1 probe. A combination of RFLP and allozyme segregation data failed to demonstrate which of twoAdh loci, both of which map to chromosome 4, was the source of the cloned gene. The product of the cloned genes has been identified unequivocally by a transient expression assay inPetunia protoplasts. We have designated this genePetunia Adh1. The expression of this gene is tightly regulated in the developing anther, where its gene product is the predominant ADH isozyme. It is anaerobically inducible in roots, stems and leaves of seedlings. The induction of enzyme activity is correlated with induction ofAdh1 mRNA.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 10 (1989), S. 452-459 
    ISSN: 0192-253X
    Keywords: Mu1 ; Mutator ; Maize alcohol dehydrogenase ; Transposable elements ; Gene expression ; Processing ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have examined effects of mutations created by transposition of the Mu1 element of maize into genes coding for Adh1 and Sh1, by means of allozyme measurements, DNA and RNA hybridization, cloning, and sequencing. From our analysis of mutant alleles we conclude that the element acts both to reduce steady-state levels of RNA and to induce aberrant processing of primary transcripts. We also conclude that genetic background can exert considerable influence in determining the degree to which Mu affects these aspects of gene expression.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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