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  • Life and Medical Sciences  (3)
  • Metabolism during Exercise  (2)
  • 1
    ISSN: 1432-2013
    Keywords: Slow Muscle Fibers ; Fast Muscle Fibers ; Plasma FFA ; Blood Lactate ; Metabolism during Exercise ; Fiber Recruitment ; Fiber Types
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The glycogen depletion pattern in human muscle fibers was followed throughout the course of prolonged exercise at a work load requiring 67% of the subjects' maximal aerobic power. Biopsy samples were taken from the vastus lateralis muscle at rest and after 20, 60, 120, and 180 (or when unable to continue at the prescribed load) min of exercise. Muscle fibers were identified as fast twitch (FT) or slow twitch (ST) on the basis of myofibrillar ATPase activity. The glycogen content of muscle samples was determined biochemically. At the end of the exercise total muscle glycogen content was very low. Glycogen was also estimated in the fibers with the PAS stain. ST fibers were the first to become depleted of their glycogen but as the exercise progressed, the FT fibers were also depleted. These data may suggest a preferential utilization of ST fibers during prolonged, intense exercise, with a secondary recruitment of FT occuring as the ST fibers became depleted of their glycogen stores.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: Muscle Glycogen ; Blood Lactate ; Fiber Types ; Blood Glucose ; Oxygen Uptake ; Liver Glycogen ; Metabolism during Exercise
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Total muscle glycogen depletion, the glycogen depletion pattern (PAS staining) in the different fiber types of skeletal muscle, and several other measures of carbohydrate metabolism were studied in rats that ran 162.2 m at varying speeds (22.5–80.5 m/min) or swam from 0.5–4 hrs. Muscle glycogen declined as an increasing function of exercise intensity during running whereas during swimming there was a near linear decline in muscle glycogen throughout the 4 hrs of exercise. Blood lactate did not increase until running speed exceeded a load that would required a VO2 of about 60% of aerobic capacity. a peak lactate of 21.15 mM occurred after the rats ran at 67m/min. Liver glycogen declined steadily at a rate of about 0.6 mg×g−1×min−1 during the first 2 hrs of swimming. During this time blood glucose was maintained at or above resting levels. During the final 2 hrs of swimming glycogenolysis in the liver declined to about 0.09 mg×g−1×min−1 and there was then a sharp decrease in blood glucose to a final value of 68.7 mg/100 ml. At low running speeds and during the first hour of swimming the greatest loss in PAS staining occured in fast-twitch-oxidative-glycolytic (FOG) and slow-twitch-oxidative (SO) fibers. At running speeds above about 50 m/min a rapid loss of PAS staining was observed in the fast-twitch-glycolytic (FG) fibers. These higher speeds are above those that should elicit VO2 max for the rat. In the late stages of swimming FG fibers also demonstrated a loss of glycogen. These data suggest that at low work intensities there is a primary reliance upon oxidative fibers for contractile activity and that a major use of anaerobic fibers only occurs at high work levels or when the aerobic fibers are depleted of glycogen during prolonged-low intensity work.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 182 (1988), S. 318-324 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The purpose of this study was to determine histologically the distribution of microspheres (MSs) (14 μm), and hence the relative distribution of blood flow, in rat plantaris muscle relative to the fiber types (fast-twitch-oxidative-glycolytic [FOG], fast-twitch-glycolytic [FG], and slow-twitch-oxidative [SO]). Three conditions were investigated: (1) preexercise standing; (2) treadmill locomotion at 15 m/min (fast walking); and (3) treadmill locomotion at 60 m/min (moderate galloping). The MS suspension (containing 1 × 106 MSs) was infused into the ascending aorta via a catheter in the carotid artery under each of the 3 conditions so that MSs were distributed to the tissues in proportion to their respective blood flows. Sections (20 m̈m) of the plantaris muscle were cut and assayed for reduced nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR) and myofibrillar adenosine triphosphatase (ATPase) activities so the fibers could be typed as SO, FOG, or FG. MSs were located in the NADH-TR sections, and the fibers next to the MSs were classified and counted. The observed numbers of fibers of each type in each condition that were adjacent to MSs were compared to the predicted number of adjacent fibers based on the assumption the MSs were randomly distributed in the tissue. This analysis demonstrated that MSs (and blood flows) were preferentially distributed to SO fibers during preexercise, to SO and FOG fibers during slow locomotion, and to FOG fibers during fast locomotion. The data support the contention that blood flow is distributed in muscles of conscious animals as functions of fiber type and exercise intensity.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 163 (1982), S. 87-98 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The distribution of Type I and Type II fibers, as determined from histochemical estimation of myofibrillar ATPase activity, was studied within and among the locomotory muscles of the forelimb, trunk, and hindlimb of three mongrel dogs. All Type II fibers had high oxidative capacities as estimated from the histochemical assay for reduced nicotinamide adenine dinucleotide tetrazolium reductase, so they were not further divided into subpopulations. Furthermore, Type I and Type II fibers had similar oxidative potentials as indicated by both histochemistry and biochemistry.Type I fiber populations ranged between 14% and 100% in the muscles sampled. The highest percentages of Type I fibers were found in deep muscles of physiological extensor groups in the arm and thigh that serve to resist gravity (antigravity muscles) when the dog is in the quadrupedal standing position. More superficial muscles in these same groups had fewer Type I fibers. The patterns of Type I fiber distribution among muscles in the antigravity groups of the forearm and leg were the opposite of those in the arm and thigh, with the more superficial muscles of the distal limb segments having more Type I fibers than the deeper muscles. In all limb segments, muscle groups that do not serve to resist gravity did not show as much intermuscular variation. Type I fiber populations in these muscles did not exceed 50%. A stratification of fiber types also existed within muscles, both in extensor and flexor groups, with the deeper portions of the muscles having more Type I fibers than the more superficial portions.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 171 (1984), S. 259-272 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The purpose of this study was to estimate the absolute and relative masses of the three types of skeletal muscle fibers in the total hindlimb of the male Sprague-Dawley rat (Rattus norvegicus). For six rats, total body mass was recorded and the following weights taken from dissection of one hindlimb: 32 individual major muscles or muscle parts, remaining skeletal musculature (small hip muscles and intrinsic foot muscles), bone, inguinal fat pad, and skin. The fibers from the 32 muscles or muscle parts (which constituted 98% of the hindlimb skeletal muscle mass) were classified from histochemistry as fast-twitch oxidative glycolytic (FOG), fast-twitch glycolytic (FG), or slow-twitch oxidative (SO), and their populations were determined. Fiber cross-sectional areas from the same muscles were measured with a digitizer. Mass of each of the fiber types within muscles and in the total hindlimb was then calculated from fiber-type population, fiber-type area, and muscle-mass data. Skeletal muscle made up 71% of the total hindlimb mass. Of this, 76% was occupied by FG fibers, 19% by FOG fibers, and 5% by SO fibers. Thus, the FG fiber type is clearly the predominant fiber type in the rat hindlimb in terms of muscle mass. Fiber-type mass data are compared with physiological (blood flow) and biochemical (succinate dehydrogenase activities) data for the muscles taken from previous studies, and it is demonstrated that these functional properties are closely related to the proportions of muscle mass composed of the various fiber types.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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