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  • 1
    Electronic Resource
    Electronic Resource
    The requirement of ArcA and Fnr for peak expression of the cyd operon in Escherichia coli under microaerobic conditions (1991)
    Springer
    Molecular genetics and genomics 226 (1991), S. 209-213 
    Details
    ISSN: 1617-4623
    Keywords: Microaerobiosis ; cyd ; arcA ; fnr ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Transcriptional regulation of cyd, encoding the cytochrome d complex for O2 scavenging, was studied in Escherichia coli by monitoring ϕ(cyd-lac) expression under atmospheres containing 0–21% O2. Peak expression of this operon occurred under microaerobic conditions. Mutations in arcA (a trans-acting regulatory gene involved in aerobic respiration) greatly lowered ϕ(cyd-lac) expression under all conditions. Mutations in fnr (a trans-acting regulatory gene involved in anaerobic respiration) shifted the pattern of ϕ(cyd-lac) expression, lowering the microaerobic and aerobic expression. In an arcA fnr double mutant, ϕ(cyd-lac) expression became insignificant, irrespective of the availability of O2. It thus appears that the expression of the ϕ(cyd-lac) operon is under dual control by the two pleiotropic activators, ArcA and Fnr, which interact to give the peak microaerobic expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273605
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The selection of a stable rat hepatoma variant with concomitant increase in ploidy and permeability to glycerol (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 94 (1978), S. 197-203 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: By repeated selection for longer survival in an isotonic solution of glycerol, a stable subline of Novikoff rat hepatoma cells has been isolated. The cells exhibit markedly increased resistances to osmotic lysis in isotonic solutions of glycerl. They are twice as large and have twice as many chromosomes as cells of the parental line. It is suggested that the osmotic stress procedure can be extended for the selection of numerous kinds of mutants and can be used as a method of analysis of membrane properties.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040940209
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Glycerol transport and phosphorylation by rat hepatocytes (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 117 (1983), S. 230-234 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The entry of glycerol into isolated rat hepatocytes appears to be catalyzed by a specific carrier. At a physiological concentration of 0.1 mM, glycerol utilization is rate limited by the permeation step. Intracellular glycerol is trapped by an excess of glycerol kinase, which has a higher apparent affinity for the substrate than that of the membrane carrier. The entry of glycerol into the hepatocytes is highly sensitive to inhibition by monoacetin and cytochalasin B, but not by DL-1,2-propanediol, erythritol, D-glucose, D-galactose, D-mannose, or D-fructose.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041170214
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    Paper (German National Licenses)
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