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Meiosis-dependent mRNA splicing of the fission yeast Schizosaccharomyces pombe mes1 + gene (1994)

Kishida, Masao ; Nagai, Tsutomu ; Nakaseko, Yukinobu ; [et al.]

Springer

Current genetics 25 (1994), S. 497-503

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ISSN: 1432-0983

Keywords: Fission yeast ; Intron ; Meiotic second division ; Splicing

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The mes1 + gene of the fission yeast Schizosaccharomyces pombe is essential for the second meiotic division. We have cloned a 1.1-kb HindIII fragment containing mes1 + by complementation from an S. pombe genomic library. Sequencing of the genomic and cDNA fragments indicates the existence of one small intron of 75 nucleotides, although both the 5′ (G/GTTAGT) and 3′ (CAG/T) intron-exon junctions deviate from the consensus sequences proposed for S. pombe. The putative translation product of the mature mes1 + mRNA is a 11-kDa protein of 101 amino acids which has no significant homology to any previously-reported proteins. Disruption of mes1 has no effect on cell growth but causes an arrest of meiosis before the second meiotic division. Northern-blot analysis revealed that mes1 + was preferentially transcribed under conditions of nitrogen starvation. When a h 90 homothallic strain was shifted to a nitrogen-deficient medium, a pre-mRNA accumulated and then was gradually processed to generate a mature mRNA. This splicing did not occur in either a heterothallic haploid strain or in a homothallic mei2 mutant strain which was defective in the initiation of meiosis. Expression of the first exon alone was not able to suppress the mes1 null allele. These results indicate that mes1 + is required for the completion of meiosis, that splicing is required for the function of the mes1 + gene, and that this splicing requires the function of the mei2 + product.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00351668

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A telomerase mutant defective in sister chromatid separation at mitosis (1997)

Nakaseko, Yukinobu ; Yanagida, Mitsuhiro

New York, NY : Wiley-Blackwell

BioEssays 19 (1997), S. 557-559

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ISSN: 0265-9247

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: The telomere is a functional domain of the chromosome, located at the extreme ends, and is essential for normal chromosome stability. Chromosomes lacking telomeres are inherited improperly, and mutations in the telomeric repeat sequences are thought to lead to senescence and possibly to cancer. The molecular mechanisms maintaining chromosomes by telomeres, however, have been unclear. Results recently reported by Kirk et al.(1) offer an insight into new telomerase function. They have identified a novel telomerase mutation that blocks sister chromatid separation in mitosis.

Additional Material: 1 Ill.