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  • 1
    ISSN: 1573-7330
    Keywords: in vitro fertilization ; human sperm ; normal sperm morphology ; sperm movement characteristics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract One hundred fourteen semen samples from Chinese males were analyzed for routine semen parameters including the semen volume, sperm count, percentage motility, and percentage normal morphology. Of these 114 samples, 54 also had movement characteristics of seminal and swim-up sperm evaluated by the computer image analyzer system (Cellsoft; Cryo Resources Co., New York). All semen samples were subjected to the swim-up procedure to harvest the motile sperm before inseminations of human oocytes. Fertilization was considered to have occurred when at least one oocyte was observed with two or more pronuclei. Semen samples were classified as infertile (0% fertilization rate;N=32) or fertile (〉0% fertilization rate;N=82) before statistical analyses. There was a significant difference (P〈0.005) in percentage normal morphology of seminal sperm between the fertile (mean±SE; 67.3±1.2%) and the infertile (59.3±2.2%) samples. The percentage normal morphology of seminal sperm correlated (r=0.3049;P〈0.002) with the fertilization rate and this parameter was selected by the multivariate stepwise discriminant analysis as the discriminator capable of predicting the fertilization rate with 57.9% accuracy. Statistical analyses of samples where sperm movement was also evaluated demonstrated that there was significant differences (P〈0.01) between the fertile (N=38) and the infertile (N=16) samples in percentage normal morphology of seminal sperm (67.8±1.8% vs 56.2±2.6%) and curvilinear velocity of swim-up sperm (89.2±3.5 vs 68.2±7.2 μm/sec). The fertilization rates correlated with the percentage normal morphology of seminal sperm (r=0.3868;P〈0.005) and velocity of swim-up sperm (r=0.3842;P〈0.005). Multivariate stepwise discriminant analysis demonstrated that these two sperm parameters in combination were capable of predicting the fertilization rate with 74.1% accuracy. Our results indicate that seminal sperm morphology, coupled with computerassisted image analysis of movement characteristics of swim-up sperm, can help to predict the outcome of in vitro fertilization of human oocytes.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 145 (1963), S. 149-155 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Enlargement of the ulnar nerve behind the medial epicondyle was noticed in museum and demonstration specimens and also in the dissection room cadavers. An investigation was carried out to study the incidence and character of the enlargement entirely from fresh autopsy cases, which included 400 ulnar nerves from 200 autopsy cadavers of Chinese subjects of all ages. The enlargement was graded into three categories  -  slight, moderate, and marked. Histological sections, 40 μthick and stained with H. & E., were examined. Twenty nerves from ten adult bodies were tested under stress for extensibility along the length of the nerve. Fortynine per cent of the ulnar nerves and 60.5% of cadavers of the total sample showed this enlargement, the incidence being greater in males than in females and slightly greater in the right limb than the left limb. Slight and moderate enlargement occurred in all ages; marked enlargement occurred only in ages over 35. The enlargement presented a normal histological picture; the enlargement was due to an increase of connective tissue present. The experimental results of extensibility along the length of the nerve showed the retro-epicondylar region, whether enlarged or not, to be more extensible than the rest of the nerve. While there seemed to be a tendency of a decrease of extensibility with enlargement, the correlation between extensibility and enlargement was insignificant statistically.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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