ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Four groups, each containing six three-month old virgin mice of the CBA strain have been used. The four groups represent different stages of the ovarian cycle, namely proestrus, estrus, metestrus, and diestrus.The uterine tissues have been both fresh frozen and fixed in a Champy solution. The fresh frozen specimens have been sectioned in a cryostat, postfixed, and used for fluorescence, polarization, and phase contrast microscopy or stained with Sudan Black, osmic acid, toluidin blue, or the PAS-technique. The specimens, fixed in the Champy solution, have been embedded in PEG and sectioned for analysis of osmophilic substance.The lipid granules of the surface epithelium are numerous in the whole cell during diestrus. In estrus, only a small basal rim of granules is left. The lipid granules are neither autofluorescent nor anisotropic.The PAS-positive secretory products are most prominent during estrus, and consist during this stage of cytoplasmic granules, a layer on the cell surface, and an irregular substance in the lumen. In diestrus, only a few cytoplasmic granules are observed.The basement membrane is thick and undulating in diestrus and metestrus, but thin and even in estrus. The present investigation has not revealed any vacuolar degeneration of the uterine epithelium nor any fading and disappearance of its basement membrane in late estrus and in metestrus as noticed by earlier workers.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091450407
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