ISSN:
1432-072X
Keywords:
Phenol oxidases
;
Lignin degradation
;
White-rot fungus
;
Sporotrichum pulverulentum
;
Phenol oxidase-less mutant
;
Phenol oxidase-positive revertant
;
Cellulase
;
Xylanase
;
Celloblose quinone oxidoreductase
;
Wood degradation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The lignin degradation abilities of wildtype, a phenol oxidase-less mutant and a phenol oxidase-positive revertant of Sporotrichum pulverulentum were compared to determine if phenol oxidase activity is necessary for lignin degradation by white-rot fungi. The phenol oxidase-less mutant was unable to degrade kraft lignin or wood. The phenol oxidase-positive revertant, however, regained the ability of the wildtype to degrade kraft lignin and all of the major components of wood. It was found that kraft lignin and lignin-related phenols decreased cellulase and xylanase production by the phenol oxidase-less mutant. Addition of highly purified laccase increased the production of endo-1,4-β-glucanase in the phenol oxidase-less mutant in the presence of vanillic acid and kraft lignin. After addition of laccase to kraft lignin agar plates, the phenol oxidase-less mutant could degrade kraft lignin. It is proposed that phenol oxidase function in regulating the production of both lignin-and polysaccharide-degrading enzymes by oxidation of lignin and lignin-related phenols when S. pulverulentum is growing on wood.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425105
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