ISSN:
1432-5233
Keywords:
Human islet density
;
Human pancreatic islet purification
;
Linear continuous density gradients
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The purification of large numbers of human pancreatic islets remains one of the limiting factors in islet transplantation. This paper describes and validates a method for accurately and reproducibly determining the density of islets and exocrine tissue in pancreatic digest on the basis of their isopycnic distribution on linear continuous density gradients. The use of this data to analyse and compare the purity of a standard 60% islet yield is described. The results obtained using such gradients will enable factors responsible for the variation in yield between pancreases to be determined and optimized, improving the results and reliability of islet purification.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00572864
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