ISSN:
1573-3904
Schlagwort(e):
Lipophilic probe
;
β2-Subunit from CD18
;
Chaperonin 10 from Rattus norvegicus
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary The solid-phase synthesis of peptides is limited by the ability to separate the target sequence from chromatographically similar deletion and truncated impurities. Earlier we have reported the development of a one-step purification procedure for Boc- and Fmoc-synthesised peptides, which involves the incorporation of a base-labile probe with enhanced chromatographic properties at the N-terminal residue of the peptidyl-resin. To prevent the coderivatisation of deletion peptides, an efficient capping procedure is required at each step of chain assembly to terminate unreacted amino groups. N-(2-Chlorobenzyloxycarbonyloxy)-succinimide (Z(2-Cl)-OSu) was found to be a highly effective capping agent for automated SPPS, because it is (i) a solid which can be dissolved when required to limit possible degradation; (ii) stable to the reagents commonly used for Boc/Fmoc chemistries; and (iii) sufficiently reactive so as not to significantly extend cycle times. We demonstrate the effectiveness of a 5 min capping cycle, using Z(2-Cl)-OSu, by synthesising several peptides ranging from 12 to 101 residues in length, by both the Fmoc and Boc chemical strategies.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00122923
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