ISSN:
1573-5036
Keywords:
Electron transport
;
Flavonoids
;
Luteolin
;
Photophosphorylation
;
Photosynthesis
;
Quercetin
;
Spinach
;
Taxifolin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary The effects of luteolin, quercetin, and taxifolin on light induced phosphorylation and electron transport in isolated, greenhouse-grown, spinach (Spinacia oleracea L.) thylakoids were investigated. Luteolin and quercetin interacted with components associated with both the ATP-generating pathway and the electron-transport pathway. However, the action of taxifolin involved only the phosphorylation pathway. Interference with the phosphorylation pathway was evidenced by the greater sensitivity of phosphorylation than oxygen uptake in coupled whole-chain electron transport, inhibition of the light-activated Mg2+-ATPase, and inhibition of the Ca2+-ATPase associated with CF1. The following order of decreasing inhibitory effectiveness was exhibited: luteolin 〉 quercetin 〉〉〉 taxifolin. On the electron-transport pathway, luteolin and quercetin interfered with the activity of the QB-protein complex as evidenced by inhibition of the partial reaction with diphenylcarbazide as the electron donor and 2,6-dichlorophenolindophenol as electron acceptor; alteration of the chlorophyll fluorescence transients; and competitive displacement of radiolabeled atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine].
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02381735
Permalink
