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  • 1
    Electronic Resource
    Electronic Resource
    Activation of cationic amino acid transport through system y+ correlates with expression of the T-cell early antigen gene in human lymphocytes (1992)
    Springer
    Pflügers Archiv 422 (1992), S. 87-89 
    Details
    ISSN: 1432-2013
    Keywords: T lymphocytes ; cationic amino acid transport ; T cell early antigen gene ; system y+ ; system y+L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Lysine influx (2 μM) into activated human B and T lymphocytes through transport systems y+ and y+L was measured. In activated T cells very substantial activation of system y+ was detected; system y+L was also activated in these cells but with a slower time course and to a smaller extent. No stimulation of either system was found in activated B cells. The time course of activation of system y+ precisely matched the expression of the T cell early antigen gene described by MacLeod et al, 1990. The functional significance of these observations with respect to L-arginine transport and nitric oxide synthesis in activated T lymphocytes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00381518
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Method for generation of human B lymphoblastoid cell lines using Epstein-Barr virus (1995)
    Springer
    Methods in cell science 17 (1995), S. 67-74 
    Details
    ISSN: 1573-0603
    Keywords: B lymphocytes ; Epstein-Barr virus ; Immortalization ; Lymphoblastoid cell line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed methodology is described for the successful immortalization of B lymphocytes to give lymphoblastoid cell lines using Epstein-Barr (EB) virus. A virus stock is prepared from B95-8 or Akata cell line, which spontaneously release high titers of virus, and the immortalizing titer of the preparation determined. B lymphocytes from fresh or cryopreserved samples are separated from the whole cell population, infected with EB virus and cultured for 4 weeks for the establishment of a lymphoblastoid cell line. The presence of EB virus encoded proteins in the immortalized cell line can be detected by indirect immunofluorescent staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00986653
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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