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  • 1
    ISSN: 1573-5176
    Keywords: brown algae ; Laminaria japonica ; fatty acids ; sterols ; β-carotene ; tocopherol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seasonal variation in major lipid constituents of nutritional importance in culturedLaminaria japonica Aresch., such as fatty acids, sterols, β-carotene and tocopherol, were investigated from December to October, the growing season. The total and saturated fatty acid contents were minimal in midsummer. Mono-unsaturated fatty acids gradually increased from late summer to autumn. The polyunsaturated fatty acid content (PUFA, (n-6) family) was maximal during warm months, while (n-3) PUFAs were most abundant during the cold months when algal thalli were very young, and decreased gradually toward October when sori had developed. Fucosterol content was maximum from February to June, but decreased steeply by nearly a half toward October, when 24-methylene cholesterol was highest although much less than fucosterol. The β-carotene and tocopherol contents were maximal from July to September and slight during the winter.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5117
    Keywords: alkali-soluble alginate ; block structure ; boiling water-soluble alginate ; brown alga ; Kjellmaniella crassifolia ; M:G ratio ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Kjellmaniella crassifolia, the edible macro-brown alga in Japan contained nearly 27% of alginates of which nearly 7% was extractable from the fronds with boiling water for 6 h and the residual alginates in the frond were almost exhaustively extracted with a dilute alkali at 60 °C for 6 h. The alginates dissolved in all these extracts with both boiling water and dilute alkali were purified by fractionation with MgCl2 and alcohol. The content of MM blocks in the boiling water-soluble alginate sample increased remarkably during heating for 6 h while that of GG blocks from the same sample decreased. In contrast, MM blocks in the alkali-soluble alginate sample decreased during 6 h heating while GG blocks continued to increase. Since the amounts of MG blocks showed slight fluctation, the M:G ratio of alginates extracted with boiling water increased towards the end of extraction whereas the reverse is true for the alkali-soluble alginates.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5117
    Keywords: lipid metabolism ; lipid classes ; Laminaria japonica ; brown algae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study to assess which environmental or developmental factors predominate in the biosynthesis of lipids of Laminaria japonica Aresch. blades was undertaken by means of 14C-labelling technique. In experiment 1, kelp blades at different growth stages were collected in different cultural seasons. In experiment 2, kelp blades of different sizes and maturity cultured simultaneously for two months in the same sea area were collected at the same time. The following results were obtained. In experiment 1, the 14C-incorporation into whole lipids was lowest in juvenile blades collected at the end of autumn and highest in blades of middle size collected in winter. However, the highest counts were incorporated in PC among complex lipid classes from all size classes of blades in both experiments 1 and 2. In experiment 2, 14C-incorporation patterns of individual lipid classes were characteristically different depending on the sizes of blades even under the same cultural condition. Thus, the biosynthesis of lipids in this kelp seems to be affected essentially by developmental factors.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5117
    Keywords: brown algae ; Laminaria japonica ; fucan ; sulphated polysaccharide ; monthly change ; molar ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Crude fucan was extracted monthly from makonbu, Laminaria japonica Areschoug, cultured from April to October at a southern site of the Hokkaido bay. The crude fucan yield tended to gradually increase from April to September, and rose markedly in October when spore formation was over. The fucans were fractionated on DEAE-Sephadex A-25 into three acidic components, A, B and C. In all the monthly extracts, fraction C was the largest. It was entirely free of laminaran and alginate, as was fraction B. Several sugars such as mannose and glucuronic acid, were identified in fraction C beside a large amount of fucose and sulphate. Only the proportion of the latter two constituents continued to increase as the alga matured to reach twice or even more than three times as much as all the other sugars in the September extract.
    Type of Medium: Electronic Resource
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